COMPARISON OF DECONTAMINATION METHODS FOR ISOLATION OF MYCOBACTERIUM SPECIES FROM THE ENVIRONMENT

  • Type: Project
  • Department: Zoology
  • Project ID: ZOO0021
  • Access Fee: ₦5,000 ($14)
  • Pages: 128 Pages
  • Format: Microsoft Word
  • Views: 206
  • Report This work

For more Info, call us on
+234 8130 686 500
or
+234 8093 423 853

ABSTRACT

The environment harbours many bacterial species, some of which include non-tuberculous mycobacteria which have recently become important in public health. Isolation of mycobacteria from the environment has not been easy because of the presence of other fast growing bacteria and fungi. For isolation of mycobacteria from the environment, decontamination methods that minimize contamination but maximize recovery of mycobacteria are needed. This study sought to compare three decontamination methods for isolation of mycobacteria from the environment. Sixty-five samples were collected from both Buruli ulcer disease endemic and non-endemic villages. Polymerase chain reaction (PCR) was done to detect the biomarker IS2404 as a first screening procedure. Direct microscopy was performed on the IS2404 positive samples and three decontamination methods were evaluated, namely; 4% NaOH/ 5% simplified OA method, 0.3% malachite green/ 0.75 g/50 ml cycloheximide/ 4% NaOH and 3% SDS/ 4% NaOH decontamination methods. Three different media with antibiotic supplementation and one without antibiotic supplementation were used for isolation of mycobacteria from the environment. Thirty-seven out of the 65 samples were positive for IS2404 marker and 5/37(13.5%) were acid-fast positive. Decontamination by NaOH/OA method gave the highest number of total tubes that confirmed mycobacterial growth (42/91, 46.1 %) and the least contamination. The medium containing PANTA-mycobactin-J (PM) was best among the four media used. Isolates obtained from this study were identified by Hain GenoType CM® line probe assay. Forty-four Mycobacterium species were identified and Mycobacterium chelonae was the most frequently isolated species. Decontamination with 4% sodium hydroxide/5% oxalic acid and L-J medium containing PANTA and mycobactin J (PM) were the most efficient in supporting the growth of mycobacteria and may be used as standard for isolating mycobacteria from the environment. 

COMPARISON OF DECONTAMINATION METHODS FOR ISOLATION OF MYCOBACTERIUM SPECIES FROM THE ENVIRONMENT
For more Info, call us on
+234 8130 686 500
or
+234 8093 423 853

Share This
  • Type: Project
  • Department: Zoology
  • Project ID: ZOO0021
  • Access Fee: ₦5,000 ($14)
  • Pages: 128 Pages
  • Format: Microsoft Word
  • Views: 206
Payment Instruction
Bank payment for Nigerians, Make a payment of ₦ 5,000 to

Bank GTBANK
gtbank
Account Name Obiaks Business Venture
Account Number 0211074565

Bitcoin: Make a payment of 0.0005 to

Bitcoin(Btc)

btc wallet
Copy to clipboard Copy text

500
Leave a comment...

    Details

    Type Project
    Department Zoology
    Project ID ZOO0021
    Fee ₦5,000 ($14)
    No of Pages 128 Pages
    Format Microsoft Word

    Related Works

    Outliers are every day problems encountered in all spheres of life. This work addresses the different methods of labelling outliers and as well highlights the applications of outliers in real life processes... Continue Reading
    Outliers are every day problems encountered in all spheres of life. This work addresses the different methods of labelling outliers and as well highlights the applications of outliers in real life processes... Continue Reading
    ABSTRACT A study was conducted to determine the presence of Salmonella species in Frozen Foods in Owerri in August, 2000. A total of 30 Food samples were collected from the different market in ten different locations in Owerri and examined for the presence of... Continue Reading
    There is a crucial need in the mainland regions of Nigeria to enhance the development of wind technology and engineering, which is essential for the designing of Wind Energy Conversion Systems (WECS). The Weibull Probability Density Function (PDF) with two parameters is widely accepted and commonly utilized for modelling, characterizing and... Continue Reading
    ABSTRACT This project work is concerned with study of the comparison of Gaussian elimination and cholesky decomposition methods to linear system of equations. In chapter one, we are concerned with linear systems and the various methods of... Continue Reading
    There is a crucial need in the mainland regions of Nigeria to enhance the development of wind technology and engineering, which is essential for the designing of Wind Energy Conversion Systems (WECS). The Weibull Probability Density Function (PDF) with two parameters is widely accepted and commonly utilized for modelling, characterizing and... Continue Reading
    Abstract Indigenous bacteria in the soil environment can produce a wide range of solvents and metabolites more efficiently. This study was carried out to screen Clostridium species isolated from the soil environment within Jos metropolis for solvent production. A total of 30 samples were collected from farmlands, nursery gardens, floral gardens... Continue Reading
    ABSTRACT The study was to determine the prevalence of M. tuberculosis in Enugu metropolis. The study was done using five areas in Enugu metropolis, Ogui Road, Onu Asata, Obiagu, Agbani Road and Okpara Avenue. Among the five areas used Agbani Road has the highest positive prevalence... Continue Reading
    ABSTRACT The study was carried out to detect Mycobacterium bovis in cattle milk and abattoir staff in Enugu. Blood samples were collected from 50 subjects at Artisan and Ogbete market abattoirs with 25 samples from each location. Fifty Milk samples were collected from cattle at Fulani settlements which comprised of 13 from Gariki, 16 from 9th Mile... Continue Reading
    ABSTRACT This research is based on the comparisons of measures of central tendency. This is to find the most appropriate measures for average, using variance for easy collection of data of middle numbers that are centrally placed. Data was collected on which the various measures of central tendency was... Continue Reading
    Call Us
    Get this work
    whatsappWhatsApp Us