Abstract This study was aimed to calculate the GFR in patient whom had diabetic mellitus and hypertensive disease to show how it effecting the GFR. Analytical study designed and conducted in the nuclear medicine department at fedil hospital, in the period from September to December (2016). The data were collected from 35 patients with renal problem where 15 were diagnosis as normal GFR patient, 10 of them were diagnosis as diabetic patient, and 10 were diagnosis as hypertensive patient. Tc99m -DTPA was administrating to the patient intravenously and Gamma camera (SPECT MULTICAM 2000”) was used to detect the count and hence GFR were calculated. The result showed that the uptake phase co-offecient for hypertensive was decrease by 6.17cont /second while uptake phase cooffecient for diabetic was decrease by 3.743 cont /second, that meaning the effect of hypertensive is more than that of diabetic. In conclusions we found there is significant difference in absorption co-offecient phase between normal and abnormal (hypertensive and diabetic) one which equal 0.007and 0.013at probability p=0.05 in hypertensive and diabetic with normal respectively done by t-test. On the other hand the secretion phase inconclusive difference these due to the hypertension and diabetic have no direct effect on secretion phase, it function of renal tubule not direct effect thought the blood vessels.