ANTIBIOTIC SENSITIVITY TESTING ON EGGS FROM LAYERS TREATED WITH ANTIBIOTIC
A RESEARCH PROJECT
ABSTRACT
Antibiotic sensitivity testing is the susceptibility of bacteria to antibiotics, it is carried out to determine which antibiotic will be most successful in treating a bacterial infections. The total mean colony count of the bacterial isolates in the two set of egg sample carried out showed the mean total bacterial count as 0.33x102 – 3.6 x 102 cfu/ml. While the antibiotics sensitivity testing of bacterial isolate isolated from two set of egg sample on four types of antibiotics showed the inhibition zone as follows Norfloxacin (≤ 13mm - ≥ 18mm) Gentamycin (≤ 17mm - ≥ 18mm); Rifampin (≤ 13mm - ≥ 18mm); Levofloxacin (≤ 18mm - ≥ 19mm).
TABLE OF CONTENTS
CONTENTS PAGE
Title page -- -- -- -- -- -- -- -- i
Certification -- -- -- -- -- -- -- ii
Dedication -- -- -- -- -- -- -- -- iii
Acknowledgement -- -- -- -- -- -- -- iv
Abstract -- -- -- -- -- -- -- -- v
List of tables -- -- -- -- -- -- -- -- vi
Table of Contents -- -- -- -- -- -- -- vii-ix
CHAPTER ONE
1.0 Introduction -- -- -- -- -- -- -- 1
1.1 Background and Statement of the Problem -- --
1.2 Objective of the Study -- -- -- -- -- --
1.3 Scope and Limitation of the Study -- -- --
CHAPTER TWO
2.0 Literature Review -- -- -- -- -- --
2.1 Poultry -- -- -- -- -- -- --
2.2 Uses of Poultry -- -- -- -- -- --
2.3 Nutrition Value of Poultry -- -- -- --
2.4 Poultry Practices -- -- -- -- -- --
2.4.1 Intensive Poultry Farming -- -- -- --
2.4.2 Free-range Poultry Farming -- -- -- --
2.5 Poultry Farming in Akwa Ibom State -- --
2.6 Antimicrobials -- -- -- -- -- --
2.7 Antimicrobials Use in the Poultry Industry --
2.7.1 Growth and Performance Enhancement -- --
2.7.2 Control of Infections and Disease -- -- --
2.8 Microflora of Eggs -- -- -- -- --
2.9 Regulation of Antimicrobial Use
in Poultry Production -- -- -- -- --
2.10 Effect of Antimicrobial Use in Poultry -- --
2.10.1 Emergence of Antibiotic
Resistance Bacteria Strains -- -- -- --
2.10.2 Transmission of Disease -- -- -- --
CHAPTER THREE
3.0 Materials and Methods -- -- -- -- --
3.1 Sample Collection -- -- -- -- -- --
3.2 Sterilization of Materials -- -- -- -- --
3.3 Media Preparation -- -- -- -- -- --
3.4 Isolation and Enumeration of Bacterial Isolates --
3.5 Characterization and identification
of Bacterial Isolates -- -- -- -- --
3.6 Gram Staining -- -- -- -- --
3.7 Biochemical Testing -- -- -- -- --
3.7.1 Catalase test -- -- -- -- -- --
3.7.2 Oxidase test -- -- -- -- -- --
3.7.3 Urease test -- -- -- -- -- --
3.7.4 Starch test -- -- -- -- -- --
3.7.5 Sugar fermentation -- -- -- -- --
3.8 Antibiogram -- -- -- -- -- --
3.8.1 Preparation of antibiotic disc -- -- -- --
3.8.2 Antibiotic sensitivity testing -- -- -- --
CHAPTER FOUR
4.0 Result and Discussion -- -- -- -- --
4.1 Result -- -- -- -- -- -- --
4.2 Discussion -- -- -- -- -- -- --
CHAPTER FIVE
5.0 Summary and Conclusion -- -- -- --
5.1 Summary -- -- -- -- -- -- --
5.2 Conclusion -- -- -- -- -- -- --
References -- -- -- -- -- -- --
