ABSTRACT
Ethanol, Bleach and Phenolics are three kinds of disinfectants which have been widely used in common laboratories. In this study, a compared experiment on these three disinfectants efficiency was conducted against Staphylococcus aureus and Pseudomonas aeruginosa using agar hole diffusion method. Different concentrations of bleach (1%, 2%, 3%, 4% and 5%) were used on both organisms. Also (50%, 60%, 70%, 85% and 95%) of ethanol as well as (5%, 10%, 20%, 25%, and 30%) Phenolics were used. Diffrences in concentrations tested was because, the original concentrations of the disinfectants differs. After 24 hours of incubation at 370C, the results showed that all the disinfectants inhibited the growth of the test organism in their concentrated forms. The diameter of zone of inhibitions were measured around each well by using a ruler in millimeters, using different concentrations, their efficacies varied. The results showed that 30% Phenolics had the best efficiency against both test organisms and 5% bleach had a better effect on Staphylococcus aureus than Pseudomonas aeruginosa, while ethanol showed least sensitivity. 70% concentration gave the highest effect on Staphylococcus aureus as compared with Pseudomonas aeruginosa.
TABLE OF CONTENTS
Title Page
Certification
Dedication
Acknowledgements
Table of Contents
List of Tables
List of Figures
List of Plates
Abstract
CHAPTER ONE
1.0 Introduction
1.1 Aims and Objectives
CHAPTER TWO
2.0 Literature Review
2.1 History of Disinfectants
2.2 About Disinfectants
2.3 Sources of Contamination of Surfaces
2.4 Types of Disinfectants
2.5 Properties of A Disinfectant
2.6 General Features of Disinfectant
2.7 General Features of the Test Organisms
2.9 Mechanism of Actions of Disinfectants against Bacteria
2.10 Resistant Action of Bacteria
2.11 Advantages and Disadvantages of Disinfectants
2.12 General Guidelines in the Use of Disinfectants
CHAPTER THREE
3.0 Material and Method
3.1 Isolation of Bacteria
3.2 Identification of Isolates
3.3 Preparation of Disinfectants
3.4 Antimicrobial Susceptibility Testing (Using Kirby Bauer diffusion Assay Well Method)
CHAPTER FOUR
4.0 RESULTS
CHAPTER FIVE
5.0 Discussion
