OPTIMIZATION AND SCREENING OF EXTRACELLULAR ENZYMES PRODUCED BY PSEUDOMONAS AERUGINOSA DURING LDPE DEGRADATION

  • Type: Project
  • Department: Environmental Science
  • Project ID: EVS0247
  • Access Fee: ₦5,000 ($14)
  • Pages: 35 Pages
  • Format: Microsoft Word
  • Views: 142
  • Report This work

For more Info, call us on
+234 8130 686 500
or
+234 8093 423 853

ABSTRACT

Plastics are synthetic polymers made by chains of carbon atoms with hydrogen, oxygen, nitrogen, Sulphur built around the carbon chains (Shah et al., 2008). These polymers are involved in every domain of our lives ever since its production began from the year 1950’s, plastics have been produced at an augmented rate to meet day to day demands of the entire world. Accumulation of plastic waste in the environment causes chemical pollution when methods like incineration are used to get rid of waste. Chemicals used during the production stage of plastic like ethylene, vinyl chloride, terephthalic acid and bisphenol which are most commonly employed in consumer based plastic products, have harmful effects on human health causing diseases like cancer, endocrine diseases, birth defects and many other health issues.

The effect of temperature has been seen to contribute immensely to the ability and rate of microbial degradation of plastics and related materials. Montazer et al., (2018) have similar result reported when they subjected pretreated low density polyethene to some of these bacteria at various temperature. This had been reported earlier by Mahalashimi et al., (2012) for Pseudomonas and Klebsiella, in which temperature was a major factor in their rate degradation of plastics and related materials.

The results obtained in this research has establish that bacteria such as Pseudomonas, Klebsiella, Salmonella, Rhizobium and Streptococcus are actively involved in the degradation of bioplastics and they produced enzymes such as cutinase, esterase, laccase, manganese, peroxidase and lipase. These enzymes are actually the ones that soften these plastics resulting in their eventual decay and putrefaction. Temperature may also play a role in the rate of the plastic degradation.

 

TABLE OF CONTENTS

COVER PAGEi

TITLE PAGEii

CERTIFICATIONiii

DEDICATIONiv

ACKNOWLEDGEMENTv

ABSTRACTvi

TABLE OF CONTENTSvii

LIST OF FIGURESxii

LIST OF TABLESxiii

LIST OF PLATESxiv

ABBREVIATIONSxv

CHAPTER ONE1

INTRODUCTION1

1.1. Background Study1

1.2. Problem Statement3

1.3. Aim of Study4

1.4. Objectives of the Study4

CHAPTER TWO6

LITERATURE REVIEW6

  2.1 Concept of Biodegradation.........................................................................6

  2.2 Polyethylene...............................................................................................7

2.2.1 Types of Polyethylene...............................................................................8

2.2.2 Methods of Degradation..........................................................................14

2.3 Mechanism of Polyethylene Degradation..................................................16


CHAPTER THREE21

METHODOLOGY21

3.0 Preparation of Seed culture...................................................................20

3.1 Procedure for Enzyme Assay................................................................20

3.1.1 PETase assay......................................................................................21


CHAPTER FOUR....................................................................................................23

RESULT..................................................................................................................23

4.0 Temperature optimization..........................................................23

4.1 Enzyme Activity.........................................................................25


CHAPTER FIVE....................................................................................................52

DISCUSSION, CONCLUSION AND RECOMMENDATIONS......................52

5.1. Discussion........................................................................................................52

5.2. Conclusion.......................................................................................................54

5.3. Recommendation............................................................................................54

REFERENCES......................................................................................................55

APPENDIX............................................................................................................55

OPTIMIZATION AND SCREENING OF EXTRACELLULAR ENZYMES PRODUCED BY PSEUDOMONAS AERUGINOSA DURING LDPE DEGRADATION
For more Info, call us on
+234 8130 686 500
or
+234 8093 423 853

Share This
  • Type: Project
  • Department: Environmental Science
  • Project ID: EVS0247
  • Access Fee: ₦5,000 ($14)
  • Pages: 35 Pages
  • Format: Microsoft Word
  • Views: 142
Payment Instruction
Bank payment for Nigerians, Make a payment of ₦ 5,000 to

Bank GTBANK
gtbank
Account Name Obiaks Business Venture
Account Number 0211074565

Bitcoin: Make a payment of 0.0005 to

Bitcoin(Btc)

btc wallet
Copy to clipboard Copy text

500
Leave a comment...

    Details

    Type Project
    Department Environmental Science
    Project ID EVS0247
    Fee ₦5,000 ($14)
    No of Pages 35 Pages
    Format Microsoft Word

    Related Works

                                          ABSTRACT The present study was to determine the prevalence of pseudomonas aeruginosa in patients using clinical samples (urine, skin lesion and pus) and environmental samples (sand, tap water, flowers). A total of thirty (30) samples were collected from patients, ten (10)... Continue Reading
                                          ABSTRACT The present study was to determine the prevalence of pseudomonas aeruginosa in patients using clinical samples (urine, skin lesion and pus) and environmental samples (sand, tap water, flowers). A total of thirty (30) samples were collected from patients, ten (10)... Continue Reading
    Abstract The emergence of multiple antibiotic resistance in bacteria and the indiscriminate use of antibiotics contribute to the dissemination of resistant pathogens in the environment which may cause problems in therapy and is a serious public health issue. This study was conducted to determine the incidence of  Pseudomonas aeruginosa  and ... Continue Reading
    ABSTRACT Ethanol, Bleach and Phenolics are three kinds of disinfectants which have been widely used in common laboratories. In this study, a compared experiment on these three disinfectants efficiency was conducted against Staphylococcus aureus and Pseudomonas aeruginosa using agar hole diffusion method. Different concentrations of bleach (1%, 2%,... Continue Reading
    ABSTRACT Ethanol, Bleach and Phenolics are three kinds of disinfectants which have been widely used in common laboratories.  In this study, a compared experiment on these three disinfectants efficiency was conducted against Staphylococcus aureus and Pseudomonas aeruginosa using agar hole diffusion method.  Different concentrations of bleach (1%,... Continue Reading
    ABSTRACT Ethanol, Bleach and Phenolics are three kinds of disinfectants which have been widely used in common laboratories.  In this study, a compared experiment on these three disinfectants efficiency was conducted against  Staphylococcus aureus and Pseudomonas aeruginosa  using agar hole diffusion method. Different concentrations of bleach... Continue Reading
    ABSTRACT Optimization is the process of transforming a piece of code to make more efficient (either in terms of time or space) without changing its output or side-effects. The only difference visible to the code’s user should be that it runs faster and/or consumes less memory. It is really a misnomer that the name implies you are finding an... Continue Reading
    ABSTRACT Optimization is the process of transforming a piece of code to make more efficient(either in terms of time or space) without changing its output or side-effects. The onlydifference visible to the code’s user should be that it runs faster and/or consumes lessmemory. It is really a misnomer that the name implies you are finding an... Continue Reading
    TABLE OF CONTENT   An Indicator Species & Identification   Description of Microcystis aeruginosa   Ecological Characteristics, Evolutionary Strategies & Ecological Class   What Makes Microcystis aeruginosa an Indicator of Disturbance   Successional Status   Agents that Promote Microcystis aeruginosa   Usefulness offs an Indicator References... Continue Reading
    TABLE OF CONTENTS CHAPTER ONE 1.0       Introduction 1.1       Enzymes 1.2       Classification of Enzymes 1.3       Factors affecting enzymes activities 1.4       Kinetic of Enzymes catalyzed reactions CHAPTER TWO 2.0       Immobilization process 2.1    Immobilized Enzymes 2.2    Immobilization techniques and... Continue Reading
    Call Us
    whatsappWhatsApp Us