+234 813 0686 500
+234 809 3423 853
info@grossarchive.com

Phytochemical Screening And Invivo Antiplasmodial Activity Of Methanol Extract Of Baobab Leaves (Adansonia Digitata)

  • Type:Project
  • Pages:47
  • Format:Microsoft Word
(Bio-Chemistry Project Topics & Materials)
ABSTRACT
Twenty-two healthy albino mice were grouped into A, B, C, D, and E. Group A had six mice while groups B – E had four mice each. The mice were infected with Plasmodium berghei and the methanol extract of the leaves of Adansonia digitata was obtained by maceration extraction method. Phytochemical screening of the plant extract revealed the presence of alkaloids, flavonoids, saponins, carbohydrates and cardiac glycosides. The infected mice Group C, D and E were orally treated with the methanol leaves extract, while Group B was orally treated with water. After twenty days of treatment, the result obtained showed that the parasite level of the mice in Group E, treated with 400mg/kg body weight of the extract was greatly suppressed, so also was that of Group B, treated with 25mg/kg body weight of lumarthem, while Group C and D treated with 100mg/kg and 200mg/kg body weight of the extract showed a moderately suppressed growth of the parasite. The mice in Group A that were untreated showed an increase in the parasitemia level. The methanol extract of Adansonia digitata possesses invivo antiplasmodial activity on Plasmodium berghei by suppressing the parasite level but appears to be dose dependent.

TABLE OF CONTENTS
CHAPTER ONE
1.0 Introduction __________________________ 1
1.1 Malaria - Background _____________________ 2
1.2 Malaria parasites __________________________ 4
1.3 Plasmodium berghei _____________________ 5
1.4 Baobab leaves (medicinal uses) ___________ 6
1.5 Aim and objectives _____________________ 7
CHAPTER TWO 
2.0 Literature review ___________________________ 8
2.1 Malaria ________________________________ 8
2.1.1 Cause ________________________________ 9
2.1.2 Life cycle ________________________________ 9
2.1.3 Signs of symptoms ___________________________ 10
2.1.4 Complications ___________________________ 10
2.1.5 Diagnosis _________________________________ 11
2.1.6 Treatment _________________________________ 11
2.1.7 Prevention _________________________________ 12
2.2 Plasmodium berghei _______________________ 13
2.2.1 Life cycle of P. berghei (asexual development) 14
2.2.2 Sexual development ______________________ 15
2.2.3 Oocyst and sporozite development ____________ 17
2.3 Baobab (Adansonia digitata) __________________ 18
2.4 Biological activity of baobab __________________ 19
2.5 Uses of baobab in traditional medicine _______ 22
2.6 Scientific classification Adansonia digitata _______ 24


CHAPTER THREE
3.0 Materials and methods _________________ 25
3.1 Materials _________________________________ 25
3.1.1 Apparatus _________________________________ 25
3.1.2 Reagents _________________________________ 26
3.2 Collection and preparation of plant material _______ 26
3.3 Plant extraction ____________________________ 27
3.4 Phytochemical screening __________________ 27
3.5 Source of experimental animals _____________ 30
3.6 Parasite source and invivo test __________________ 30
3.7 Animal group and invivo anti-plasmodial test ___ 31
3.8 Blood examination _______________________ 31
3.9 Calculation of percentage parasitemia and 
percentage yield ____________________________ 32

CHAPTER FOUR
4.0 Result s _________________________________ 33

CHAPTER FIVE
5.0 Discussion _________________________________ 36
5.1 Conclusion _________________________________ 37
5.2 Recommendation ____________________________ 38
Reference _________________________________ 39 

Phytochemical Screening And Invivo Antiplasmodial Activity Of Methanol Extract Of Baobab Leaves (Adansonia Digitata)

Share This

Details

Type Project
Department Bio-Chemistry
Project ID BCH0115
Price ₦3,000 ($9)
No of Pages 47 Pages
Format Microsoft Word

500
Leave a comment...

    Details

    Type Project
    Department Bio-Chemistry
    Project ID BCH0115
    Price ₦3,000 ($9)
    No of Pages 47 Pages
    Format Microsoft Word

    Related Works

    ABSTRACT Twenty-two healthy albino mice were grouped into A, B, C, D, and E. Group A had six mice while groups B – E had four mice each. The mice were infected with Plasmodium berghei and the methanol extract of the leaves of Adansonia digitata was obtained by maceration extraction method. Phytochemical screening of the plant extract revealed... Continue Reading
    ABSTRACT Antibiotic resistance has become one of the major problems facing humanity. The need for new antimicrobials has been increased dramatically. Plants are considered as a major sources of new antibiotics due to the presence of phytochemicals. In Nigeria and other developing countries medicine plant materials have been used to treat various... Continue Reading
    ABSTRACT The research investigated the extract and phytochemical screening of (pumpkin leaves). The plant leaves were collected dried grinded and extracted using water by a cold extraction (maceration) method. The filtrated was concentrated and their subjected to phytochemical screening... Continue Reading
    ABSTRACT Azadirachta indica, commonly known as Neem, has attracted worldwide prominence in recent years as a cynosure of modern medicine owing to its wide range of medicinal properties. This study was carried out to investigate the phytochemical properties and antioxidant activity of the multi-solvent (methanol, ethanol and hexane) extracts of the... Continue Reading
    ABSTRACT was studied. The leave was observed to be highly rich in polyphenols, flavonoid, reducing compounds and alkaloids. Glycosides, saponins and tanins were present in small amount, there was absence of Phlobotanins,... Continue Reading
    PHYTOCHEMICAL SCREENING OF COCONUT WATER EXTRACT ABSTRACT Medicinal plants are the local heritage with global importance plants and plant based medicaments are the basis of many of the modern pharmaceuticals we use today for our various ailments. The research investigated the photochemical screening of coconut water. Coconut water was obtained... Continue Reading
    ABSTRACT The methanol extract of Ocimum basilicum (Scent leaf) was greenish in colour and increases in concentration through evaporation process which turns the extract to thick and dark green colouration. The percentage parasitaemia growth increases consecutively for seven (7) days with 7th day result having the highest parasite growth of 18.73%.... Continue Reading
    Activation of caspase 3&9 by methanol extract of Gloriosa superba  ABSTRACT Mitochondrion is the key regulator in cellular energy homeostasis and plays a central role in determining cell apoptotic process; it is therefore regarded as a vital target for cancer chemotherapy.   Cancer is obviously one of the most common areas where demand for... Continue Reading
    ABSTRACT Lannea acida is a plant known to have various medicinal and therapeutic properties. The phytochemicals or secondary metabolites and antibacterial activity of its root and stem bark were studied in this research work. The phytochemical screening of the plant was carried out using standard methods of analysis and these investigations... Continue Reading
    ABSTRACT Inflammation is a complex biological response of vascular tissue to harmful stimuli such as pathogen, damage cells or irritants. The urgency generated by in increased rate of stroke, atheroselerosis attributed to prolong use of cyclooxygenase-1 and 2 inhibitors have acceralated anti-inflammatory drug research over the last decade while... Continue Reading