EFFECTS OF AQUEOUS ETHANOLIC LEAF EXTRACTS OF MUCUNA PURIENS AND CHROMOLAENA ODORATA ON ISOLATED UTERINE SMOOTH MUSCLE OF THE ALBINO RAT

  • Type: Project
  • Department: Veterinary Medicine
  • Project ID: VEM0027
  • Access Fee: ₦5,000 ($14)
  • Pages: 107 Pages
  • Format: Microsoft Word
  • Views: 478
  • Report This work

For more Info, call us on
+234 8130 686 500
or
+234 8093 423 853

ABSTRACT

The contractile activity of M. pruriens and the relaxatory property of C. odorata was investigated using in vitro methods in the rat. M. pruriens caused a dose-dependent increase in uterine muscle contraction with a maximum peak in amplitude with 0.57 mg/ml of the extract and an EC50 of 2.7 mg/ml. The contraction was unaffected by atropine sulphate (0.042 µmol), but abolished by isoprenaline (0.06-0.23 µmol), salbutamol (0.012-0.4 µmol) and adrenaline (16 nmol).Uterine muscle contractions were enhanced by propranolol (1 µmol) in a dose- dependent manner. Prazosin (0.069-0.14 µmol) and atipamezole (3.3-13.7 nmol) were not able to abolish contractions stimulated by the extract however, 0.2 µmol of cyproheptadine caused 86% abolition of the extract –induced uterine contraction. The mechanism of Ca2+ mobilization in uterine muscle cells by M. pruriens isolated was also investigated in the rat. Uterine muscle contractions were significantly attenuated (100%; P < 0.01) in Ca2+-free physiological salt solution and in solutions containing verapamil (0.007- 0.14 mmol). Contractions stimulated by M. pruriens were unaffected by amiloride (1.1-2.1 µmol) in Ca2+-containing media. It is concluded that M.pruriens causes uterine muscle contraction by mobilizing external Ca2+ through a voltage-dependent Ca2+ channel. On the other hand, C. odorata caused a dose –dependent decrease in spontaneous contracting uterine tissue, giving an EC50 of 1.0 mg/ml. C. odorata was also able to decrease uterine contractions stimulated by acetylcholine ( 0.16-0.47 µmol), carbachol (1.6-7.8 µmol), oxytocin ( 2.6 x10-4 nmol), CaCl2 (0.1-1 mmol) -induced contractions and KCl (30 mmol) depolarized tissue. Propranolol (1 µmol) had no effect on C. odorata –induced relaxation. It is concluded that C. odorata causes relaxation via blockade of voltage operated calcium channels.

EFFECTS OF AQUEOUS ETHANOLIC LEAF EXTRACTS OF MUCUNA PURIENS AND CHROMOLAENA ODORATA ON ISOLATED UTERINE SMOOTH MUSCLE OF THE ALBINO RAT
For more Info, call us on
+234 8130 686 500
or
+234 8093 423 853

Share This
  • Type: Project
  • Department: Veterinary Medicine
  • Project ID: VEM0027
  • Access Fee: ₦5,000 ($14)
  • Pages: 107 Pages
  • Format: Microsoft Word
  • Views: 478
Payment Instruction
Bank payment for Nigerians, Make a payment of ₦ 5,000 to

Bank GTBANK
gtbank
Account Name Obiaks Business Venture
Account Number 0211074565

Bitcoin: Make a payment of 0.0005 to

Bitcoin(Btc)

btc wallet
Copy to clipboard Copy text

500
Leave a comment...

    Details

    Type Project
    Department Veterinary Medicine
    Project ID VEM0027
    Fee ₦5,000 ($14)
    No of Pages 107 Pages
    Format Microsoft Word

    Related Works

    HEPATOTOXICITY OF THE ETHANOLIC EXTRACTS OF Enantia chlorantha ROOT ON WISTAR ALBINO RATS ABSTRACT The root of Enantia chlorontha was assayed for its hepatotoxicity effect. Extacts from the root were made using 95% ethanol. The root extracts were administered orally to 15 wistar albino rats and varying doses. 5 rats were kept as control group. The... Continue Reading
    ABSTRACT This study was conducted to determine the phytochemical constituents and to evaluate the anti diarrheal effect of the ethanolic stem bark extract of Acacia sieberiana in albino rats. The rats were grouped into five different groups; two groups were treated with water and loperamide (a standard drug) which serve as negative and positive... Continue Reading
    CHAPTER ONE 1.0  GENERAL INTRODUCTION 1.1 Introduction Grains constitute the most important staple food for the growing population in most parts of the world and are usually stored to provide food reserve and seeds for planting (Niber, 1994). Maize is a very important crop of the world after wheat and rice (Purseglove, 1992). Maize (Zea mays)... Continue Reading
    Abstract Piper  guineense   ( Uziza  in Igbo) is a medicinal plant whose leaves are commonly used as spices.   This study is to investigate the effects of the  ethanolic  leaf extracts of  P. guineense   on the h istology of the liver , liver biom arker enzymes  of  paraquat -induced Thirty (30) adult male  Wistar  rats w eighing... Continue Reading
    ABSTRACT The ethanolic leaves extracts of Hymenocardia acida was screened for anti-diabetic activity based on folklore and little or no work cited in literature. Hymenocardia acida (Family: Hymenocardiaceae) has a very high anti-diabetic potentials which appeared comparable to the control glibenclamide in alloxan induced diabetes mellitus. The... Continue Reading
    ABSTRACT The ethanolic leaves extracts of Hymenocardia acida was screened for anti-diabetic activity based on folklore and little or no work cited in literature. Hymenocardia acida (Family: Hymenocardiaceae) has a very high anti-diabetic potentials which appeared comparable to the control glibenclamide in alloxan induced diabetes mellitus. The... Continue Reading
    The medicinal values of some species of Mistletoes ( Tapinanthus globiferus ) growing in the West African sub-region have been reviewed along with some considerations of their chemistries and local uses. Studies carried out revealed that mistletoes showed hypotensive, hypoglycemic etc. This present study was aimed at investigating some effects of... Continue Reading
    The medicinal values of some species of Mistletoes ( Tapinanthus globiferus ) growing in the West African sub-region have been reviewed along with some considerations of their chemistries and local uses. Studies carried out revealed that mistletoes showed hypotensive, hypoglycemic etc. This present study was aimed at investigating some effects of... Continue Reading
    ABSTRACT Uvaria chamae P. Beauv is known to have various medicinal and therapeutic properties. The Biochemical and toxicological effects of the leaf extract were assessed in this study using Albino wistar rats. Acute toxicity test was performed on the rats to determine the LD50.Sub acute toxicity test was also carried out by oral administration of... Continue Reading
    ABSTRACT was studied. The leave was observed to be highly rich in polyphenols, flavonoid, reducing compounds and alkaloids. Glycosides, saponins and tanins were present in small amount, there was absence of Phlobotanins,... Continue Reading
    Call Us
    whatsappWhatsApp Us