ABSTRACT
A study was undertaken to determine typical concentration of airborne bacteria and fungi (microflora) in Enugu State University of Science and Technology (ESUT) in the tropical rainforest environment of Nigeria. Aerial sampling was conducted at  various faculties and  departments each day. The air samples were collected  thrice daily. Concentrations of airborne microflora exceeded  available local guidelines  for classroom,  lobby, library and offices, but not in the restricted places. The occupant density was the key factor influencing the levels of airborne microflora, depending on the particular location within the school. The concentrations of airborne bacteria and fungi in the four different school units varied from department to department. The bacterial population ranges from 3.0cfu/m3 to 70.0cfu/m3, with the highest bacterial population recorded in the classroom. The fungal  population ranges from 6.0cfu/m3 to 44.7cfu/m3, while the highest fungal population was recorded in the classroom  and officeses. The microflora of the human body (skin, gastrointestinal tracts, respiratory tract) and the opportunistic pathogens where all isolated.  The Escherichia   coli  and Bacillus, and the fungal Isolates included, Aspergillus, Penicillium, Mucor, and Candida  spp. are the concentration of airborne microflora recorded in the school environment, specifically in the classroom was significantly different from other places.

TABLE OF CONTENTS
Contents                                                                                           pages
Title page                                                                                              i
Certification                                                                                          ii
Dedication                                                                                            iii
Acknowledgement                                                                               iv
Abstract                                                                                                v
Table of Content                                                                                   vi
Introduction                                                                                          1
Objective Of The Studies                                                                     3
Literature  Review                            
Diseases Associate With Poor Sanitation                                            4
Diseases & Microorganisms Associated With Poor Sanitation            6
Effect Of Poor Sanitation                                                                     7
Poor Sanitation That Affect The Environment                                     8
Isolation Of Microorganism From Air                                                   8
Environmental Factors That Affect Environment  Air                           9
Different Method Used In Isolation Of Microorganism                        11

Material And Method                                                                           12
Air Sampling For Microbiological Examination                                   13
Pure Culture Preparation                                                                    14
Gram Staining For Bacterial Isolates                                                  15
Gram Staining For Fungi Isolates                                                       16
Result         
Microbial Load In Study Areas                                                           17   
Composition And Frequency Of Air Bone Microflora                         19
Mixed Colonies of Bacterial Isolates                                                  20
Mixed Colonies of Fungil Isolates                                                      21
Discussion                                                                                         22
Conclusion                                                                                         24
Recommendation                                                                               25
Reference                                                                                           26