Evidences have implicated free radicals or reactive oxygen species in mercuric chloride-induced nephrotoxicity. Diphenyl diselenide (PhSe)2, a simple and stable organoselenium compound, has been proposed as a good candidate for pharmacological and therapeutic purposes. This particular study was designed to investigate the protective effect of (PhSe)2 against mercuric chloride-induced nephrotoxicity.
In this study, 44 female Wistar rats with weights ranging from 125g and 180g were randomly divided into 5 groups. Control rats in groups 1 and 2 were administered normal saline and/or corn oil. Group 3 animals were treated with (PhSe)2 alone while those in Groups 4 and 5 were challenged with HgCl2. Group 5 rats were additionally co-treated with (PhSe)2. Mercuric chloride and (PhSe)2 were given orally at a specific dose of 5 mg/kg/day. Integrity of kidney was assessed by urea and creatinine levels and oxidative stress indices were also determined. Results were analysed using analysis of variance (ANOVA) with significance level set at p<0.05.
Rats exposed to HgCl2 showed no significant decrease in body or organ (kidney) weight when compared with the control. There was a significant increase in the level of urea in the plasma of rat exposed to HgCl2 when compared with the controls while the level of creatinine remained unaffected. The group treated with (PhSe)2 alone also had elevated urea level. However co-treatment of HgCl2 and (PhSe)2 resulted in enhanced kidney function. Moreover, there was also significant increase in the levels of malondialdehyde (MDA), Hydrogen peroxide (H2O2), reduced Glutathione (GSH) and activities of catalase,Glutathione peroxidase (Gpx), Glutathione-S-transferase (GST), myeloperoxidase (MPO) on exposure to HgCl2. These were markedly reversed by the administration of the organoselenium compounds.
The result of this study indicates that DPDS may be effective in attenuating HgCl2-induced nephrotoxicity.
THE POTENTIAL PROTECTIVE EFFECTS OF DIPHENYL-DISELENIDE (PHSE)2 ON MERCURIC CHLORIDE-INDUCED NEPHROTOXICITY IN FEMALE WISTAR RATS
ABSTRACT Evidences have implicated free radicals or reactive oxygen species in mercuric chloride-induced nephrotoxicity. Diphenyl diselenide (PhSe)2, a simple and stable organoselenium compound, has been proposed as a good candidate for pharmacological and therapeutic purposes. This particular study was designed to investigate the protective... Continue Reading
Abstract The effect of Shea butter rich diet on gentamicin induced nephrotoxicity in rats was determined. Thirty (30) male and female albino rats grouped into 6 (normal, negative, positive, 10%, 20% and 30% Shea butter rich diet groups respectively) of 5 rats each were used for the study. Phytochemical analysis was carried out on the Shea... Continue Reading
ABSTRACT INTRODUCTION: Various researches had shown that Nigella sativa has diverse characteristics some of which include bitter, pungent, aromatic, appetizer, stimulant, diuretic, emmenagogue, galactagogue, anthelminthic, acrid, thermogenic, carminative, anodyne, deodorant, digestive, constipating, sudorific,... Continue Reading
The overall objective of this study is to scientifically evaluate the claimed fertility enhancing potential and to assess the metabolic effect of the ethanolic extract of Sphenocentrum jollyanum pierre (Menispermaceae) root using male wistar rats as model. The specific objectives include the following · To determine the proximate and... Continue Reading
CHAPTER ONE 1.0 INTRODUCTION Procreation is an important moral and religious issue and aphrodisiacs are sought to ensure both male and female potency. Sexual potency is part of the male ego, the anxiety and the humiliation that are associated with a declining sexual ability are common to all cultures. That is why the popularity of aphrodisiacs has... Continue Reading
ABSTRACT Plants have long been a source of nutrition and therapy to man. The medicinal value of these plants lies in some chemical substances that produce a definite physiological action in the human body. This study evaluated the oxytocic potential of methanol extract of Sida acuta on female albino rats in vitro. Oxytocin was used as a standard... Continue Reading
This research project studied the effect of ethanolic leaf extract of Dialium guineense Willd (Fabaceae) on oral glucose tolerance in mice and alloxan-induced diabetic female rats. The ethanolic leaf extract was prepared by marcerating 500g of the dried, milled leaves of D. guineense in 3L of 96% ethanol in a suitable sized plastic bucket/bowl... Continue Reading
TABLE OF CONTENT CHAPTER ONE 1.0 INTRODUCTION 1.1 AIMS AND OBJECTIVES OF THE RESEARCH CHAPTER TWO 2.0 LITERATURE REVIEW 2.1 SCIENTIFIC CLASSIFICATION OF SENNA TORA 2.2 DESCRIPTION OF SENNA TORA 2.3 SOME COMMON NAMES OF SENNA TORA 2.4 GEOGRAPHICAL DISTRIBUTION OF SENNA TORA 2.5 GROWTH REQUIREMENTS OF SENNA TORA 2.6 PROPAGATION OF SENNA TORA 2.7... Continue Reading
ABSTRACT The present study was conducted in Ekpoma, headquarters of Esan West Local Government area of Edo state. The aim of the study was to determine the effect of marijuana on the heart of wistar rats. The study involved twenty four growing wistar rats having weights between 250g-275g divided into 4 groups; control and groups 1-3, comprising... Continue Reading