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Antimicrobial Activities of Oil Extracts of Piper Guineense and Xylopia Aethiopica on Some Microbial Pathogens of Food

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  • Pages:65
  • Format:Microsoft Word
(Food Technology Project Topics & Materials)

ABSTRACT

Oils extracted from the fruits of two Africa spices- Piper guineense and Xylopia aethiopica were evaluated for their antimicrobial activities using Escherichia coli, Salmonella sp, Aspergillus sp, Bacillus cereus, Penicillum commune and Rhizopus stolonifer as test isolates. Well-in-agar, disc diffusion and dilution susceptibility testing methods were used to assay for their antimicrobial properties. For the bacterial isolates the antimicrobial activity of the oils increased with increase in concentration. B. cereus was more susceptible to the oils than other isolates with zones of growth inhibition measuring 19.5 mm and 18.0 mm for P. guineense and X. aethiopica respectively. However, the disc diffusion method produced lower inhibitory effect on the isolates. The MIC values ranged from 8.0 mg/ml – 16.0 mg/ml while MBC values ranged from 16-32mg/ml. Percentage inhibition against concentration of the oils revealed that percentage inhibition increased with increase in concentration ranging from 91.28% as observed on B. cereus produced by P. guineense to 96.86% on Salmonella sp. produced by X. aethiopica. The antifungal activities of the oils showed moderate to high activities on the isolates. This shows that essential oils of Piper guineense and Xylopia aethiopica has both bactericidal and fungicide effects which can be exploit in disease prevention and control as well as food preservation.

Key words: Antimicrobial activities, Oil extract, Piper guineense, Xylopia aethiopica, Escherichia coli, Salmonella sp, Aspergillus sp.


TABLE OF CONTENT

Title page

Certification

Dedication

Acknowledgements

Abstract

Table of content 

CHAPTER ONE

1.0 Introduction
1.1 Background of study

1.2 Statement of problems

1.3 Objectives of the study

1.4 Justification
1.5 Scope 

CHAPTER TWO

2.0 Literature review
2.1 Essential oils
2.2 Indigenous spices
2.3 Medicinal uses of indigenous spices
2.4 Antimicrobial effects of extracts from indigenous spices on 
common bacterial and fungi 

CHAPTER THREE

3.0 Materials and methods

3.1 Materials 

3.2 Methods 

3.2.1 Sample preparation

3.2.2 Extraction of oils from the samples

3.2.3 Media preparation

3.2.3.1 Preparation of nutrient agar

3.3.2 Preparation of eosin methylene blue agar

3.3.3 Preparation of salmonella shigella agar

3.3.4 Preparation mueller hinton agar

3.3.5 Preparation of potato dextrose agar

3.3.6 Characterization of bacterial isolates

2.5.1 Microscopic identification

2.5.2 Biochemical tests

3.4 Antimicrobial susceptibility testing

3.4.1 Well in agar method 

3.4.2 Disc diffusion method

3.5 Dilution susceptibility testing

3.5.1 Determination of minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) of oils from spices. 

3.6 Antifungal assay

3.7 Data analysis 

CHAPTER FOUR

4.0 Results and discussion

4.1. Antimicrobial susceptibility testing

4.2. Dilution susceptibility testing (dst)

4.3. Antifungal activity of piper guineense and xylopia aethiopica against selected molds 

5.0 Conclusion and recommendation

5.1 Conclusion
5.2 Recommendations
5.3 Contribution to knowledge References

Appendix 1

Appendix 2

Appendix 3

Appendix 4 


LIST OF TABLE

Table 2.1 Antibacterial activity of the water extracts from the spices, mm 13

Table 2.2 Antibacterial activity of ethanol extracts from the spices, mm 14

Table 3.1 Antifungal activity of ethanolic extracts from the spices, mm 14

Table 4.1. Minimum inhibitory concentration of piper guineense and xylopia aethiopica against selected isolates 33

Table 4.2: Antifungal activity of piper guineense and xylopia aethiopica against selected molds 


LIST OF FIGURES

Figure 1(A): Fresh Piper guineense Seeds 7

Figure 1(B): Dried Piper guineense Seeds 7

Figure 2(A): Fresh Xylopia aethiopica Fruit 8

Figure 2 (B): Dried Xylopia aethiopica Fruit 8

Figure 4.1: Antimicrobial Susceptibility Testing of Isolates Using Well in Agar Diffusion Method. 30

Figure 4.2: Antimicrobial Susceptibility Testing of Isolates using the Disc Diffusion Method 31

Figure 4.3: Graph of % Inhibition against Concentration Showing Activity of Piper guineense and Xylopia aethiopica against

Escherichia Coli. 34

Figure 4.4: Graph of % Inhibition against Concentration Showing Activity of Piper guineense and Xylopia aethiopica against

Bacillus cereus. 

Figure 4.5: Graph of % Inhibition against Concentration Showing Activity of Piper guineense and Xylopia aethiopica against Salmonella spp. 

Antimicrobial Activities of Oil Extracts of Piper Guineense and Xylopia Aethiopica on Some Microbial Pathogens of Food

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Details

Type Project
Department Food Technology
Project ID FTE0126
Price ₦3,000 ($9)
No of Pages 65 Pages
Format Microsoft Word

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    Details

    Type Project
    Department Food Technology
    Project ID FTE0126
    Price ₦3,000 ($9)
    No of Pages 65 Pages
    Format Microsoft Word

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