ANTIMICROBIAL ACTIVITIES OF OIL EXTRACTS OF PIPER GUINEENSE AND XYLOPIA AETHIOPICA ON SOME MICROBIAL PATHOGENS OF FOOD

  • Type: Project
  • Department: Food Technology
  • Project ID: FTE0126
  • Access Fee: ₦5,000 ($14)
  • Pages: 65 Pages
  • Format: Microsoft Word
  • Views: 798
  • Report This work

For more Info, call us on
+234 8130 686 500
or
+234 8093 423 853

ABSTRACT

Oils extracted from the fruits of two Africa spices- Piper guineense and Xylopia aethiopica were evaluated for their antimicrobial activities using Escherichia coli, Salmonella sp, Aspergillus sp, Bacillus cereus, Penicillum commune and Rhizopus stolonifer as test isolates. Well-in-agar, disc diffusion and dilution susceptibility testing methods were used to assay for their antimicrobial properties. For the bacterial isolates the antimicrobial activity of the oils increased with increase in concentration. B. cereus was more susceptible to the oils than other isolates with zones of growth inhibition measuring 19.5 mm and 18.0 mm for P. guineense and X. aethiopica respectively. However, the disc diffusion method produced lower inhibitory effect on the isolates. The MIC values ranged from 8.0 mg/ml – 16.0 mg/ml while MBC values ranged from 16-32mg/ml. Percentage inhibition against concentration of the oils revealed that percentage inhibition increased with increase in concentration ranging from 91.28% as observed on B. cereus produced by P. guineense to 96.86% on Salmonella sp. produced by X. aethiopica. The antifungal activities of the oils showed moderate to high activities on the isolates. This shows that essential oils of Piper guineense and Xylopia aethiopica has both bactericidal and fungicide effects which can be exploit in disease prevention and control as well as food preservation.

Key words: Antimicrobial activities, Oil extract, Piper guineense, Xylopia aethiopica, Escherichia coli, Salmonella sp, Aspergillus sp.


TABLE OF CONTENT

Title page

Certification

Dedication

Acknowledgements

Abstract

Table of content 

CHAPTER ONE

1.0 Introduction
1.1 Background of study

1.2 Statement of problems

1.3 Objectives of the study

1.4 Justification
1.5 Scope 

CHAPTER TWO

2.0 Literature review
2.1 Essential oils
2.2 Indigenous spices
2.3 Medicinal uses of indigenous spices
2.4 Antimicrobial effects of extracts from indigenous spices on 
common bacterial and fungi 

CHAPTER THREE

3.0 Materials and methods

3.1 Materials 

3.2 Methods 

3.2.1 Sample preparation

3.2.2 Extraction of oils from the samples

3.2.3 Media preparation

3.2.3.1 Preparation of nutrient agar

3.3.2 Preparation of eosin methylene blue agar

3.3.3 Preparation of salmonella shigella agar

3.3.4 Preparation mueller hinton agar

3.3.5 Preparation of potato dextrose agar

3.3.6 Characterization of bacterial isolates

2.5.1 Microscopic identification

2.5.2 Biochemical tests

3.4 Antimicrobial susceptibility testing

3.4.1 Well in agar method 

3.4.2 Disc diffusion method

3.5 Dilution susceptibility testing

3.5.1 Determination of minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) of oils from spices. 

3.6 Antifungal assay

3.7 Data analysis 

CHAPTER FOUR

4.0 Results and discussion

4.1. Antimicrobial susceptibility testing

4.2. Dilution susceptibility testing (dst)

4.3. Antifungal activity of piper guineense and xylopia aethiopica against selected molds 

5.0 Conclusion and recommendation

5.1 Conclusion
5.2 Recommendations
5.3 Contribution to knowledge References

Appendix 1

Appendix 2

Appendix 3

Appendix 4 


LIST OF TABLE

Table 2.1 Antibacterial activity of the water extracts from the spices, mm 13

Table 2.2 Antibacterial activity of ethanol extracts from the spices, mm 14

Table 3.1 Antifungal activity of ethanolic extracts from the spices, mm 14

Table 4.1. Minimum inhibitory concentration of piper guineense and xylopia aethiopica against selected isolates 33

Table 4.2: Antifungal activity of piper guineense and xylopia aethiopica against selected molds 


LIST OF FIGURES

Figure 1(A): Fresh Piper guineense Seeds 7

Figure 1(B): Dried Piper guineense Seeds 7

Figure 2(A): Fresh Xylopia aethiopica Fruit 8

Figure 2 (B): Dried Xylopia aethiopica Fruit 8

Figure 4.1: Antimicrobial Susceptibility Testing of Isolates Using Well in Agar Diffusion Method. 30

Figure 4.2: Antimicrobial Susceptibility Testing of Isolates using the Disc Diffusion Method 31

Figure 4.3: Graph of % Inhibition against Concentration Showing Activity of Piper guineense and Xylopia aethiopica against

Escherichia Coli. 34

Figure 4.4: Graph of % Inhibition against Concentration Showing Activity of Piper guineense and Xylopia aethiopica against

Bacillus cereus. 

Figure 4.5: Graph of % Inhibition against Concentration Showing Activity of Piper guineense and Xylopia aethiopica against Salmonella spp. 

ANTIMICROBIAL ACTIVITIES OF OIL EXTRACTS OF PIPER GUINEENSE AND XYLOPIA AETHIOPICA ON SOME MICROBIAL PATHOGENS OF FOOD
For more Info, call us on
+234 8130 686 500
or
+234 8093 423 853

Share This
  • Type: Project
  • Department: Food Technology
  • Project ID: FTE0126
  • Access Fee: ₦5,000 ($14)
  • Pages: 65 Pages
  • Format: Microsoft Word
  • Views: 798
Payment Instruction
Bank payment for Nigerians, Make a payment of ₦ 5,000 to

Bank GTBANK
gtbank
Account Name Obiaks Business Venture
Account Number 0211074565

Bitcoin: Make a payment of 0.0005 to

Bitcoin(Btc)

btc wallet
Copy to clipboard Copy text

500
Leave a comment...

    Details

    Type Project
    Department Food Technology
    Project ID FTE0126
    Fee ₦5,000 ($14)
    No of Pages 65 Pages
    Format Microsoft Word

    Related Works

    ABSTRACT This work were carried out on the phytochemcial analysis of xylopia aethiopica (ude fruit). The extract of the plant sample were prepared by soaking 5g of grinded sample in 100ml of water, ethanol and n-hexane respectively for 24 hours and filtered into conical flask using filter... Continue Reading
    Plants have been utilized for medicinal reasons since the dawn of time. Pharmacologists and organic chemists have synthesized a wide range of intriguing chemical compounds from medicinal plants in recent decades, which have shown to be quite useful in the practice of optometry and medicine. There is now an increase in global demand for the use of... Continue Reading
    ABSTRACT Garlic (Alliums Sativum) and Ginger (Zingiber officinale) is hardy perennial of Asiatic origin, belonging to the plant family liliaceae. They are grown in northern Nigeria as medicine for both human and animals. They are primarily used for seasoning and also for its medicinal property. Anti-microbial activities of garlic and ginger were... Continue Reading
    TABLE OF CONTENT Title page……………………………………………………………………..i Certification………………………………………………………………….ii                    Dedication …………………………………………………………………..iii       ... Continue Reading
    ANTIMICROBIAL ACTIVITIES OF SELECTED PLANTS (BITTERLEAF, UTAZI, AND BITTERKOLA LEAF)EXTRACT’S AGAINST FISH PATHOGENIC BACTERIA TABLE OF CONTENT   CHAPTER ONE 1.1 introduction 1.2 objectives the study references CHAPTER TWO 2.1 ethnobotanical 2.1.1 medicinal plants 2.2 bitterleaf 2.2.1 antibacterial properties of bitterleaf 2.3 utazi 2.3.1... Continue Reading
    Aquaculture has been a growing activity for the last 20 years worldwide and this impressive development has been attended by some practices potentially damaging to animal health. The bacterial infections are considered the major cause of mortality in aquaculture. Among the common fish pathogens, A. hydrophila and Y. ruckeri as gram-negative and S.... Continue Reading
    ANTIMICROBIAL ACTIVITIES OF SELECTED PLANTS (BITTERLEAF, UTAZI, AND BITTERKOLA LEAF)EXTRACT’S AGAINST FISH PATHOGENIC BACTERIA TABLE OF CONTENT CHAPTER ONE 1.1 introduction 1.2 objectives the study references CHAPTER TWO 2.1 ethnobotanical 2.1.1 medicinal plants 2.2 bitterleaf 2.2.1 antibacterial properties of bitterleaf 2.3 utazi 2.3.1... Continue Reading
    CHAPTER ONE 1.1 INTRODUCTION Plants have provided a source of inspiration for novel drug compounds, as plant derived medicines have made large contributions to human health and wellbeing. Plant extracts have been used for a wide variety of purposes for many thousands of years (Jones, 1996). The antimicrobial activity of plant oils and extracts has... Continue Reading
    CHAPTER ONE 1.1 INTRODUCTION Plants have provided a source of inspiration for novel drug compounds, as plant derived medicines have made large contributions to human health and wellbeing. Plant extracts have been used for a wide variety of purposes for many thousands of years (Jones, 1996). The antimicrobial activity of plant oils and extracts has... Continue Reading
    ABSTRACT The insecticidal effects of Piper guineense and Pirimiphos-methyl was evaluated on cowpea. The treatments used were P. guineenseat 0.2, 0.4, 0.6, 0.8, and 1g as well as Pirimiphos-methyl at 0.1g per 20g of cowpea seeds, each laid out in Complete Randomized Design (CRD) in the laboratory. Results indicated that P. guineense at 1g ... Continue Reading
    Call Us
    Get this work
    whatsappWhatsApp Us