+234 813 0686 500
+234 809 3423 853
info@grossarchive.com

THE ANTI-INFLAMMATORY ACTIVITY OF Crateva adansonii DICHLOROMETHANE FRACTION

  • Type:Project
  • Pages:59
  • Format:Microsoft Word
(Bio-Chemistry Project Topics & Materials)
ABSTRACT
Crateva adansonii is a medicinal herb commonly used in parts of Africa because of the side effects of Non-Steroidal Anti-inflammatory Drugs (NSAID), like heart diseases and kidney failure. Inflammation is a major public heart issue in the world but treatment is becoming complex because of the side effects of anti-inflammatory pharmaceutical drugs. Hence the need for alternative drug is highly required. This research work investigated the anti-inflammatory activity of dichloromethane fraction of methanol extract of Crateva adansonii stem bark, using rodent model. Adult Swiss albino rats (110-200g) of either sex were randomlydivided into 5 groups of 4 animals each. Groups 2, 3, 4, and 5 received different doses of the extract (300mg, 500mg, 700mg, and 900mg) in 3% v/v tween 80 administered intraperitonally respectively. Control group-1 received volume of 3%v/v tween 80 and standards group received 100mg/kg Ibuprofen. One hour later acute inflammation was induced by injection of 0.1ml of undiluted egg albumin into the sub planter of the right hand paw of rats. The volume of the paw was measured by mercury displacement before and at 0.5, 1, 1.5, 2, 2.5 & 3 hours after egg albumin injection, while the standard was measured at internals of one hour, for up to 4 hours. Edema formation was assessed in terms of the difference in the zero time per volume of the injected pair and its volume at the different time after egg albumin injection. For each dose of extract, percentage inhibition of edema was calculated percentage inhibition. Result show that Crateva adansonii may have anti-inflammatory effects. This finding supports the use stem bark of Crateva adansonii in not only traditional medicine for the treatment of inflammation.

TABLE OF CONTENT
Title page
Approval page
Dedication
Acknowledgment
Abstract
Table of Content

CHAPTER ONE
1.0 Introduction
1.1 Crateva adansonii
1.2 Research Aim & Objectives

CHAPTER TWO
2.1 Definition of Inflammation
2.1.1 What is Anti-inflammation? Types of Inflammation (Acute and Chronic) -
2.1.2 Steroid and non-steroidal anti-inflammatory drugs
2.1.3 Immune selective anti-inflammatory derivative
2.1.4 Pain
2.1.5 Long effect of inflammation
2.1.6 Ice Treatment of Inflammation
2.1.7 Nutritional sources of anti-inflammatory compounds
2.2 Vascular event in inflammation-
2.2.1 Vasoconstriction
2.2.2 Vasodilation
2.2.3 Vascular permeability
2.3 Cellular event
2.3.1 Leukocytes migration as specific
Hemoral/cellular immunity
2.4 Mediators of inflammation
2.4.1 Cell derived mediators of inflammation
2.4.2 Histamine
2.4.3 Cytokines
2.4.4 Serotonin (5-hydroxytryptamine)
2.4.5 Platelet-Activating factor (PAF)
2.4.6 Arachidonic Acid (AA
2.4.7 Free radicals as mediators of inflammation
2.4.8 Nitric Acid (NO)
2.4.9 Reactive Oxygen Species (ROS)
CHAPTER THREE
3.1 Materials, equipments and apparatus  - -
3.2 Chemical, reagents and practical technique- 
3.2.1 Methanol
3.2.2 N-Hexane
3.2.3 Ethyl acetate
3.2.4 Dichloromethane
3.3 Methodology
3.3.1 Collection and preparation of plant
3.3.2 Extraction and fractionation of plant materials
3.4 Phytochemical Analysis
3.5 Column chromatography
3.6 Thin layer chromatography
3.7 How to run TCL plate

CHAPTER FOUR
4.0 Experimental result
4.1 Tabular and graphical representation of effect of anti-inflammatory effect of Crateva adansonii dichloromethane extract-  -
4.2 Phytochemical analysis of extract (result)    -

CHAPTER FIVE
5.0 Discussion
5.1 Conclusion
REFERENCE
APPENDIX I
APPENDIX II
APPENDIX III
THE ANTI-INFLAMMATORY ACTIVITY OF Crateva adansonii DICHLOROMETHANE FRACTION

Share This

Details

Type Project
Department Bio-Chemistry
Project ID BCH0080
Price ₦3,000 ($9)
No of Pages 59 Pages
Format Microsoft Word

500
Leave a comment...

    Details

    Type Project
    Department Bio-Chemistry
    Project ID BCH0080
    Price ₦3,000 ($9)
    No of Pages 59 Pages
    Format Microsoft Word

    Related Works

    ABSTRACT Crateva adansonii is a medicinal herb commonly used in parts of Africa because of the side effects of Non-Steroidal Anti-inflammatory Drugs (NSAID), like heart diseases and kidney failure. Inflammation is a major public heart issue in the world but treatment is becoming complex because of the side effects of anti-inflammatory... Continue Reading
    ABSTRACT Crateva adansonii is a medicinal herb commonly used in parts of Africa because of the side effects of Non-Steroidal Anti-inflammatory Drugs (NSAID), like heart diseases and kidney failure. Inflammation is a major public heart issue in the world but treatment is becoming complex because of the side effects of anti-inflammatory... Continue Reading
    ABSTRACT Inflammation is a complex biological response of vascular tissue to harmful stimuli such as pathogen, damage cells or irritants (Ryan & Majno, 1983). The urgency generated by increased rate of stroke,... Continue Reading
    ABSTRACT Inflammation is a complex biological response of vascular tissue to harmful stimuli such as pathogen, damage cells or irritants (Ryan & Majno, 1983). The urgency generated by increased rate of stroke, atherosclerosis attribute due to prolonged use of cyclooxygenase-1 and Cyclooxygenase- 2 inhibitors have accelerated anti-inflammatory... Continue Reading
    ABSTRACT Inflammation is a complex biological response of vascular tissue to harmful stimuli such as pathogen, damage cells or irritants. The urgency generated by in increased rate of stroke, atheroselerosis attributed to prolong use of cyclooxygenase-1 and 2 inhibitors have acceralated anti-inflammatory drug research over the last decade while... Continue Reading
    ABSTRACT This research investigated the phytochemical and anti-inflammatory properties of methanol extract of Crateva adansonii stem bark. Although several edible and non-edible plants parts are used in inflammatory treatment, many record has been found of the use of Crateva adansonii stem bark. For this research, fresh stem bark of Crateva... Continue Reading
    ABSTRACT This research investigated the phytochemical and anti-inflammatory properties of methanol extract of Crateva adansonii stem bark. Although several edible and non-edible plants parts are used in inflammatory treatment, many record has been found of the use of Crateva adansonii stem bark. For this research, fresh stem bark of Crateva... Continue Reading
    ABSTRACT This research investigated the phytochemical and anti-inflammatory properties of methanol extract of Crateva adansonii stem bark. Although several edible and non-edible plants parts are used in inflammatory treatment, many record has been... Continue Reading
    ABSTRACT Inflammation is a complex biological response of vascular tissue to harmful stimuli such as pathogen, damage cells or irritants. The urgency generated by in increased rate of stroke, atheroselerosis attributed to prolong use of... Continue Reading
    ABSTRACT Citrus maxima (shaddock) is a medicinal plant used in Asia, and in some parts of Africa, because they are rich in flavonoids and some other phytoconstituent which contributed to their wide range of biological activities ranging from anti-inflammatory activity, antioxidant activity and anti tumor activity. Inflammation is the biochemical... Continue Reading