+234 813 0686 500
+234 809 3423 853


  • Type:Project
  • Chapters:5
  • Pages:65
  • Format:Microsoft Word
(Microbiology Project Topics & Materials)



This project work is aimed at examination of jollof rice served in hotels for pathogenic organisms and to find out possible pathogenic organisms in the food. Samples were collected from the hotels and were brought to the laboratory for analysis. The sample were homogenized and serial dilution of the sample was made out using 5ml sterile pipette and test tubes. The dilution were the cultured on different media, macconkey agar, and Nutrient agar, discrete bacteria colonies were observed and  each colony was gram stained, the bacteria isolation were staphylococcus aureus, bacillus, Escherichia coli, these micro organism isolated are pathogenic and are toxin when ingested in contamination food.




Background of the study                                                                               

Aim and objective                                                                              

Significance of study                                                                         


Scope of study                                                                                               

Limitation of study                                                                            



Literature review.                                                                                        

Composition of rice and its requirement                                            

Source of contamination                                                                    

Pathogenic bacterial organism that are responsible for contamination of Jollof rice                                                                                                 

Some micro organism responsible for jollof rice spoilage                  

Jollof  rice spoilage

Factors that influence  microbiological activity of food                    

Prevention of food pathogenic organisms     


Material and methods                                                                                    

Sample collection                                                                               

Media used and sterilization method                                                 

Isolation methods n                                                                           

The serial dilution technique                                                              

Plating  and incubation condition                                                      

Gram reaction                                                                        






Recommendation.                                                                                           References   


1.0              INTRODUCTION


The intention of food safely is to prevent food poisoning (the transmission of disease through food and to maintain the who lesomeness of the food product through all stages of processing, until it is finally extern. No one doubts the importance of food in our lives. All active living organisms must have a constant serviced of energy  this  energy may be supplied by materials a cell or organism has stored internally (e.g  our fat  or carbohydrate) or it may come from an external source in the environment food (Nutrition) supplies two major component of life, ENERGY and the chemical  building blood of life, (Bor).

Energy is required for the various enzymatic reaction that require an input of energy for the reaction, the catalyze for example the movement of the muscles on our legs during a race or in their intestine as we digest our intest meal on to draw air into lungs for breathing all requires energy.

Food  also supplies the structural material required for living organisms to make new macromolecules for repair of damage structure  or for new construction such as the manufacturing of off springs,. Apart from it vital function of substaing life, food is referred to as labours of pathogenic  organisms, if not prepared in a tidy and clean environment. This has been recognized as a problem in some restinants in Enugu, concerned with providing proper food for consumers.

Pathogenic organisms  exist in two major categories. INTOXICATIONS –INFECTION. Intoxication is a result of ingesting toxins produced by microbes that have grown on the food prior to it being eaten. Infection is the result of the food serving as a forniute that carries an infection pathogen deep into the juicy recesses of a body where it is able to gain a foot . generally food pathogenic organism results from contamination of food and the subsequent growth of food pathogenic organisms.

Food poisoning out breaks are of recognized by the sudden  on set of  illness with a shout period of    time among many individual who have eaten or drunk one or more food (Jollodf rice) in common. Single cases are difficult to identify unless in botulism for example there are distinct symptom. Food pathogenic organism may be one of the most common cause of actuate illness, yet cases and difficult to identify vnlesssas in Botulision for example 

There are distant Symptom. Food pathogenic organism may be one of the most common causes of acute illness. Yet cases and outbreaks are generally under recognized and under-requited.   The number of bacterial present : food (jolloff rice) may be used to determined whether or not the food (jolloff rice ) has been handled correctly. Sources, Vehicles and Reuters of continuations


                  Man, Rawfoods, Insects, animals

                           Rodents, Dust, Soil.


Food or Head










                  High   Risk  Foods.


Fig I

Some disease that are spread by bacteria that enters the body in food (jolloff rice) can multiply at an amazing rate when they are provided with warmth and moisture, (especially at room temperature) Our food can become an ideal home for then. Clean food (jolloff rice ) can be contaminated by bacterial from four main sources.

*   The people present in the workplace and their clothing.    

·                     other  food that is already contaminated.

·                     Dirty kitchen or work premises

·                     Insect and vermin.

Some time, harmful bacterial  pass directly from the source  of  high risk food, but usually they rely on other things to transfer them to food. These  things are called  vehicles. Ihekoronye A. I. And Ngoddy P.O (1979).

Indirect  contamination using an intermediate  vehicle is the most common e.g the movement  of bateria from the intestine of a food handler to food via their hands after using the toilet. Where contamination  is via for example a culting board, this is known is  cross contamination,  the path that bacteria use to more from the sources to the food is know is the Route.

The privation of pathogenic organism in food, in most cases of food poisoning chain of events takes place and if we are to reduce the incidence of illness, the chain must be broken.

Food poisoning bacteria






Give time and


High risk food





There  are different ways of breaking the food poisoning chain.

*          Protecting food  (Jolloff rice) form contamination .

*          Preventing any bacteria present in food from multiply.

*          Destory those  bacteria that are present in the food. The problem of food sanitation.   

·                     Inspecting all food and  washing fruit and vegetable before preparation.

·                     Using good personal hygiene practice always.

·                       Not coupling or sneezing over or around the food.

·                     Keeping covered as much as possible in destroy those bacteria with in food (Jollof rice) An adequate cooking food ensuring that a minimum internal cooking temperature of 800c is reacted. The heating processing such as pasteurization sterilization or caming. A combination of a suitable temperature and sufficient time is always required to destroy bacteria. The time and temperature required depends on the particular organism (e.g spores of clostridium perfringen are much more  heat resistant than salmonella bacteria).

In food safety programmes the hazard analysis critical control point (HACC?), this is recommended that every food business adopt the HACCP approach to identify all potential hazards and control them before they result in problems.

In setting up a HACCP system will involve the following

·                     Set up a HACCP team of those people who fully understand the product.

·                     Draw up flow chart that define all stages in the preparation process, from raw materials though to consumption or sale.

·                     Identify all potential hazards (eg physical chemical bacterial) e.t.c

·                     Identify the citial point consider preventive measures and decide which are needed to eliminate or  reduce poetical hazard to acceptable levels.

1.2              AIM AND OBJECTIVE

This is to find out possible pathogenic organism found in food (Jollof rice) sold to Top Rink Hotel and presidential hotel to examine and identify micro organism (bacteria) associated with  Jollof rice (food) sample collected from these hotels in Enugu. And to determines the bacteria load growth.

1.3              SIGNIFICANCE OF STUDY.

The signifcance of the work is to enable us improve the hygiene of food (Jolloff rice) served and a good knowledge of a safe food handling practice suitable light colour protection clothing to be worn and it, will also enable  us to be aware of various pathogenic organism and the infection they transmit through   food (Jollof rice). As result of this the prevention of contamination of food is to be adlered to, this can be achieved by:

·                     keeping high risk food at temperature that inhibits the growth of bacterial (i.e our of the damager Zone.) food should be kept below 40c in a refigecated unit an about 700c in a suitable warming unit.

·                     Ensuing  that during preparation food is in the danger zone for as short a time as possible high risk food must not be left sitting out at room temperature.

·                     Using suitable preservation such as salt and sugar.

·                     Using various packing method like gas flushing or vacuum packing.

1.4              hypothesis

Ho          Presence of non- pathogenic organism in the Jollof rice served.

H1          Presence of pathogen organism in the Jollof rice served.

1.5              SCOPE OF STUDY

This project work is based on the identification examination of pathogenic bacterial organism associated with (Jollof rice) served in the following hotels, Top rink and presidential hotel all in Enugu town and the cavsatire organism include  staphylococcus auvious, salmonella, clostridia per fringers, Escherichia coli, the modern problem to food satiation

1.6              STATEMENT OF PROBLEM

Pathogenic organism are organism that are referred to as specific health hazard associated with gas to intestinal distribute resulting from the consumption of food containing toxins. The  victim suffering from abdominal pain and diarrheas with more vomiting than diarrheas usually manifest the illness, which when untreated in time result to the does not result to death it had  led to unnecessary expenses in seeking medical advice. This has now made me be interested on how we can  improve on our food lygiene to avoid contamination.


Share This


Type Project
Department Microbiology
Project ID MCB0098
Price ₦3,000 ($9)
Chapters 5 Chapters
No of Pages 65 Pages
Format Microsoft Word

Leave a comment...


    Type Project
    Department Microbiology
    Project ID MCB0098
    Price ₦3,000 ($9)
    Chapters 5 Chapters
    No of Pages 65 Pages
    Format Microsoft Word

    Related Works

    FROM STORED PAP (OGI) ABSTRACT The microbial changes which took place during the steeping and storage of pan (ogi) was studied. The steeped water had an initial PH of 6.8 which latter reduced to 4.9 at the end of steeping. The bacterial number for the steeped water ranged from 4.7x104 to 3.2 x 107... Continue Reading
    . ABSTRACT           Onion is associated with micro-organisms which are capable of causing spoilage. Onions with rots were examined microscopically. A solution of it was made and the serial dilution of 1:10-1­­­­­­­­­­­­­­­ to 1:1-5  were made... Continue Reading
    ABSTRACT Onion is associated with micro-organisms which are capable of causing spoilage. Onions with rots were examined microscopically. A solution of it was made and the serial dilution of 1:10-1¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬ to 1:1-5 were made from 10ml of the onions... Continue Reading
    (A CASE) STUDY OF HOTEL PRESIDENTIAL ABSTRACT Going through the present investigation of problems and prospects of management of Hotels presidential many findings and results were made in the service and finance oriented organizations The result postulated that prospects of management Hotels effects... Continue Reading
    CHAPTER ONE INTRODUCTION 1.1 Background to the Study Previous to the era of industrial revolution, the pattern and method of salesmanship was both crude and cumbersome chiefly because the gap between the productive and consumption sectors of society was small and manageable. However, with the present complication and sophistication among both the... Continue Reading
    (A CASE STUDY OF UNIVERISTY OF UYO, HOSTELS)   CHAPTER ONE INTRODUCTION 1.1   BACKGROUND TO THE STUDY According to Okoh (2004), Nigeria has the biggest university system in sub-Saharan Africa with 114 accredited tertiary institutions. More than 50% of these facilities have over 20,000... Continue Reading
    ABSTRACT The question as to whether or not internal audit has an important role to play, has been generating a lot of controversies over years, especially, among hotel and restaurant owners. This question that seemed to be yet half way answered, gave rise to carrying out this... Continue Reading
    ABSTRACT This project work was carried out to investigate how rice production is financed in Edo State a case study of Owan East Local Government Area, Nigeria. This is due to the fact that rice is a widely consumed crop in Nigeria, in the view of farming, for farmers to carry out their farming operation, government and agricultural banks should... Continue Reading
    Abstract         Flooding has been described as one of the most important environmental factors determining the distribution of salt marsh plants. We studied the impact of five water levels on the germination and establishment of the invasive cord-grass spartina deniflora. Plant house experiment was carried out to determine the effects of time... Continue Reading
    THE ROLE OF PLANTS  IN THE TREATMENT OF DISEASES CAUSED BY MICRO-ORGANISMS BASED IN THE NATURAL PRODUCTS  CHAPTER ONE INTRODUCTION 1.1 BACKGROUNG OF STUDY 1.1.1 Phyllantus This is a genus of the family Euphorbiaceae. It was first identified in Central and Southern India in 18th century. It is called carry me seed, stone-breaker, wind breaker,... Continue Reading