+234 813 0686 500
+234 809 3423 853
info@grossarchive.com

COMPARATIVE STUDY OF THE EFFECT OF ETHANOLIC EXTRACT OF LONCHOCARPUS CYANESCENS (ELU) AND DIALIUM GUINEENSE (ICHEKU) ON THE LIPID PROFILE OF STREPTOZOTOCIN INDUCED DIABETIC ALBINO RATS

  • Type:Project
  • Chapters:5
  • Pages:50
  • Methodology:Scientific
  • Reference:YES
  • Format:Microsoft Word
(Science Lab Technology Project Topics & Materials)
COMPARATIVE STUDY OF THE EFFECT OF ETHANOLIC EXTRACT OF Lonchocarpus cyanescens (ELU) AND Dialium guineense (ICHEKU) ON THE LIPID PROFILE OF STREPTOZOTOCIN INDUCED DIABETIC ALBINO RATS
ABSTRACT

This study was carried out to determine the effect of ethanoic extract of Lonchocarpus cyanescens and Dialium guineense on the lipid profile of streptozotocin induced albino rat. The rats were randomly separated into six groups of five rats each in different cages as follows Group A: rats orally administered 200mg/kg body weight of D. guineense extract daily (normal control). Group B: induced streptozotocin diabetic rats and 200mg/kg body weight of D. guineense extract daily. Group C: rats orally administered 200mg/kg body weight of L. cyanescens extract daily (normal control). Group D: induced streptozotocin diabetic rats and 200mg/kg body weight of L. cyanescens extract daily Group E: induced streptozotocin diabetic rats administered 5mg/kg body weight of gliben clemide daily (positive control). Group F: rats fed on feed and water only (standard control). All the groups have free access to feed and drinking water while the rat feed use in the study is the growers mash of vital feed limited, Jos, Plateau State, Nigeria. The body weight of the rats were taken at weekly intervals with electronic weighing balance, while the experiment lasted for 28 days. The study lasted for four weeks. From the result of the analysis it was observed that the weight of the induced rats administered with extracts of L. cyanescens and D. guineense have a significant increase in weight and a significant decrease (P˂0.05) in blood glucose level. The result of the serum lipid profile shows no significant effect on both the control and the group administered with L. cyanescens and D. guineense. In diabetic conditions, there is increase in blood glucose level which is usually accompanied by an increase in plasma cholesterol, TAGs, LDL and decrease in HDL. Under normal conditions insulin activates the enzyme lipoprotein lipase, which hydrolyzes triglycerides. However, from the result of the study, ethanoic extract of L. cyanescens and D. guineense show no significant antihyperglycaemic activities on the streptozotocin induced albino rat.
TABLE OF CONTENT
CHAPTER ONE
INTRODUCTION
1.1 Background Study                        
1.2 Aim and Objective of the Study                     
1.3 Specific Objective of the Study                 
1.4 Problem Statement                               
CHAPTER TWO
LITERATURE REVIEW
2.1 Description of Lonchocarpus cyanescens            
2.2 Taxonomy of Lonchocarpus cyanescens                   
2.3 Ecology, Growth and development of Lonchocarpus cyanescens      
2.4 Origin and Geographic Distribution of Lonchocarpus cyanescens    
2.5 Uses of Lonchocarpus cyanescens                    
2.6 Chemical Properties of Lonchocarpus cyanescens       
2.7 Botanic Description of Dialium guineense                
2.8 Taxonomy of Dialium guineense                
2.9 Ecology and Distribution of Dialium guineense                 
2.10 Uses of Dialium guineense                    
2.11 Health Benefits of Dialium guineense                   
2.12 Chemical Composition of Dialium guineense                 
2.14 Overview of Diabetes                   
2.15 Mechanism of Diabetes Occurrence               
2.20 Lipid profile in diabtes                    
CHAPTER THREE
MATERIALS ANDMETHOD
3.1 Material                                            
3.1.1 Plant Materials                                  
3.1.2 Equipment                             
3.1.3 Chemical and Reagent                        
3.2 Method                                    
3.2.1 Plants Sample Collection               
3.2.2 Extraction of Plant Materials                        
3.2.3 Blood Glucose Determination                        
3.2.4 Experimental Design                        
3.2.5 Collection of Blood Sample from animals for Biochemical Analysis    
3.3 Blood Glucose Level                               
3.4 Serum Lipid Profile                                
3.5 Statistical Analysis                           
CHAPTER FOUR
RESULT PRESENTATION
 4.1 Mean Weight of the Rat                            
4.2 Result of the Glucose Level                        
Result of the Serum Lipid Profile                          
CHAPTER FIVE
DISCUSSION AND CONCLUSION
5.1 Discussion                            
5.2 Conclusion                         
5.3 Recommendation                           
Reference                                     
LIST OF FIGURES
Figure 1: detached part of Lonchocarpus cyanescens             
Figure 2: detached part of Dialium guineense                     

LIST OF TABLES
Table 1: Mean Weight of the Rat                 
Table 2: Glucose Level                                 
Table 3: Serum Lipid Profile                            
   ABBREVIATIONS
HDL         =     High density lipoproteins
VLDL     =     very low density lipoproptein
LDL         =     low density lipoproptein
SPSS         =     Statistical Package for Social Science
TAG         =     Triglycerides
CHOL     =     Cholesterol
DM         =     Diabetes Melitus
CAD         =    Coronary Artery Disease
CHAPTER ONE
INTRODUCTION
1.1    Background of the Study
Diabetes is a disorder of carbohydrate, protein, and fat metabolism and can represent an absolute insulin deficiency, impaired release of insulin by the pancreatic β cells, inadequate or defective insulin receptors, or production of inactive insulin. Diabetes mellitus can be classified into two main types, type 1 and type 2, with type 1 resulting from the body’s failure to produce insulin, and requires one to be injected with insulin. Type 2 diabetes mellitus describes a condition of fasting hyperglycemia that occurs despite the availability of insulin (Cooke and Plotnick, 2008).
Diabetes mellitus is a major challenge for health care systems around the world (González-Villalpando et al., 2008). Clinically, diabetes mellitus is one of the most important risk factors for cardiovascular diseases. Currently, more than 150 million people worldwide are suffering from this disease and it is expected that the number of them reaches 366 million in 2030 (Wild et al., 2014). Insulin deficiency causes higher metabolization of free fatty acid and can cause disorder in lipid metabolism. The lipid abnormalities are prevalent in diabetes mellitus because insulin resistance or deficiency affects key enzymes and pathways in lipid metabolism (Taskinen, 2012). Micro-vascular and macro-vascular complications, including cardiovascular disease (CVD), retinopathy, nephropathy, and neuropathy, occur due to chronic uncontrolled hyperglycemia in diabetics (Folli et al., 2011). It has been proposed that the composition of lipid particles in diabetic dyslipidemia is more atherogenic than other types of dyslipidemia. In diabetes the associated hyperglycemia, obesity and insulin changes highly accelerate the progression to atherosclerosis (Regmi et al., 2009).
There is evidence that each of these dyslipidemic features is associated with increased risk of cardiovascular disease, the leading cause of death in patients with type 2 diabetes. Numerous studies have demonstrated an association between LDL size or density and coronary artery disease (CAD) (Pierre et al., 2013).
Medicinal plants have been used for centuries as remedies for human diseases and offer a new source of biologically active chemical compounds. Medicinal plants are the richest bio-resources of drugs of traditional medicinal systems, modern medicines, nutraceuticals, food supplements and folk medicines (Muhammad, 2015).  Plant like Dialium guineese and Lonchocarpus cyanescens has been reported to possess both medicinal and nutritional properties capable of anit-diabetic effect (Feng et al., 2011).
Velvet tamarind (Dialium guineense) is a woody plant that occurs in the rain forest region of West Africa. It grows up to 15m high with dark green glossy leaves, each measuring 6cm to 8cm long and 2.5cm wide at the widest part of the leaf. The bark, roots and leaves of Dialium guineense has been reported to have medicinal properties and are used for the treatment of variety of health problems. The fruits of the plant are chewed by most women in Southeastern Nigeria to improve lactation and check genital infection. The leaves and stem bark are also used as folklore remedies for the treatment of infections such as diarrhoea, severe cough, bronchitis, wound, stomachaches, malaria, fever, jaundice, ulcer and diabetes. The flour of Dialium guineense pulp can be incorporated in infant foods to enhance appetite and provide remedy for the problem of scurvy and micro nutrient deficiencies. Extracts from Dialium guineense plants have been shown to possess both anti-mutagenic and molluscicidal activities (Adewuyi et al., 2012).
Lonchocarpus cyanescens also known as “ALU” is a deciduous scan dent shrub. The plant has alternate leaves, flat fruits which are 1-5 seeded, oblong pod pointed at both ends. The aerial parts yield an indigo, which is a useful colourant for cloth dyeing in West Africa since ancient times. The plant is utilized in traditional medicine, bioactivity effects of Lonchocarpus cyanescens have been demonstrated in its anti-inflammatory, anti-arthritic, antidiabetic and its relief on ulcer. It has some additional pharmacological properties including antiviral, antifungal, anti-protozoal, and antibacterial activities (Iwu and Anyanwu, 2012).

1.2 Aim of the Study
The aim of the research is to identify the effect of ethanoic extract of Dialium guineese (icheku) and Lonchocarpus cyanescens (elu) on streptozotocin induced diabetes in order to determine its potency in management of diabetes.
1.3 Specific Objectives of the Study
1. To evaluate the effect of ethanolic extract of Dialium guineese (icheku) and Lonchocarpus cyanescens (elu) on the lipid profile (high density lipoprotein, cholesterol and triglyceride) of streptozotocin induced diabetic albino rat.
2. To evaluate the effect of ethanolic extract of Lonchocarpus cyanescens and Dialium guineense on blood glucose level of streptozotocin induced albino rat.
1.4 Statement of Problem
Diabetes is a disorder that has affected many people in the world at large. It affects mostly the elderly ones. The drugs available for treatment and of management diabetes have shown varied and ambient side effects. It is a case to be concern with as it concerns development of drugs that have minimal side effect.

COMPARATIVE STUDY OF THE EFFECT OF ETHANOLIC EXTRACT OF LONCHOCARPUS CYANESCENS (ELU) AND DIALIUM GUINEENSE (ICHEKU) ON THE LIPID PROFILE OF STREPTOZOTOCIN INDUCED DIABETIC ALBINO RATS

Share This

Details

Type Project
Department Science Lab Technology
Project ID SLT0158
Price ₦3,000 ($20)
Chapters 5 Chapters
No of Pages 50 Pages
Methodology Scientific
Reference YES
Format Microsoft Word

500
Leave a comment...

    Details

    Type Project
    Department Science Lab Technology
    Project ID SLT0158
    Price ₦3,000 ($20)
    Chapters 5 Chapters
    No of Pages 50 Pages
    Methodology Scientific
    Reference YES
    Format Microsoft Word

    Related Works

    COMPARATIVE STUDY OF THE EFFECT OF ETHANOLIC EXTRACT OF Lonchocarpus cyanescens (ELU) AND Dialium guineense (ICHEKU) ON THE LIPID PROFILE OF STREPTOZOTOCIN INDUCED DIABETIC ALBINO RATS ABSTRACT This study was carried out to determine the effect of ethanoic extract of Lonchocarpus cyanescens and Dialium guineense on the lipid profile of... Continue Reading
    This research project studied the effect of ethanolic leaf extract of Dialium guineense Willd (Fabaceae) on oral glucose tolerance in mice and alloxan-induced diabetic female rats.  The ethanolic leaf extract was prepared by marcerating 500g of the dried, milled leaves of D. guineense in 3L of 96% ethanol in a suitable sized plastic bucket/bowl... Continue Reading
    ABSTRACT Diabetic mellitus is a metabolic disorder resulting from a defect in insulin secretion , insulin action or both . The deficiency of insulin leads to chronic hyperglycemia with disturbances of carbohydrate , fats and protein metabolism . Kigelia Africana is a sausage tree known to have medicinal values. The aim of this work is to evaluate... Continue Reading
    Anti-diabetic and Anti-oxidant potentials of Aqueous Extract of Eucalyptus Globulus in experimentally induced diabetic rats.... Continue Reading
    TITLE PAGE THE EFFECT OF HYDROALCOHOLIC EXTRACT OF AFRAMOMUM MELEGUETA ON SUPEROXIDE DISMUTASE ACTIVITY IN ALLOXAN INDUCED DIABETIC WISTAR ALBINO RATS BY EBELEME   EBENEZER NAU/ 2012474175 SUBMITTED TO THE DEPARTMENT OF APPLIED BIOCHEMISTRY FACULTY OF BIOSCIENCES IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF A BACHELOR OF SCIENCE... Continue Reading
    In recent years, there has been profound interest in the role of oxidative stress in the precipitation of pre- diabetic condition (1) and there are reports of oxidative stress increasing in patients with diabetes mellitus (2). Oxidative stress results when reactive forms of oxygen are produced faster than they can be safely neutralized by the body... Continue Reading
    Diabetes mellitus commonly known as diabetes is a group of metabolic disorder characterized by high blood sugar level over a prolonged period (WHO,2014) This research is aimed at investigating effect of solanum macrocapon and Anti-diabetic drug glibenclamide on some blood parameters of alloxan induced diabetic rats. The effects of four eggplant... Continue Reading
    Abstract Background: Evaluation of the medicinal uses of plants remains a growing field of research. This study investigated the effects of Eucalyptus globules on alloxan -induced diabetic rats. Materials and Methods: Thirty male albino rats were randomized into 5 groups (n=6).Group A served as the control and was maintained on standard rat chow... Continue Reading
    Call Us
    Get this work
    whatsappWhatsApp Us