ISOLATION, CHARACTERIZATION AND IDENTIFICATION OF BACTERIA FROM ORGANIC WASTE

  • Type: Project
  • Department: Microbiology
  • Project ID: MCB0258
  • Access Fee: ₦5,000 ($14)
  • Chapters: 5 Chapters
  • Pages: 48 Pages
  • Methodology: Scientific
  • Reference: YES
  • Format: Microsoft Word
  • Views: 1.3K
  • Report This work

For more Info, call us on
+234 8130 686 500
or
+234 8093 423 853
ABSTRACT
The isolation, identification and characterization of bacteria from organic waste were carried out. Sawdust waste (plant organic waste source) and abattoir waste water (animal organic waste source) samples were collected. The media used were nutrient agar for total aerobic plate count, McConkey agar for coliform count and cellulolytic media for cellulolytic count. The pour plate method was employed. Colonial morphology, gram staining and biochemical tests were used for the identification of the bacteria. The total aerobic plate for the sawdust waste ranged from 2.33 x 107 to 8.2 x 106, coliform count ranged from 7.6 x 106to 1.7 x 106Cfu/g and cellulolytic count ranged from 4.7 x 106 to 2.6 x 106Cfu/g while for abattoir waste water, total aerobic plate count, coliform count and cellulolytic count ranged from 1.03 x 107 to 7.1 x 106Cfu/ml, 5.8 x 106 to 1.2 x 106Cfu/ml and 3.7 x 106 to 1.3 x 106Cfu/ml respectively. The bacteria isolated from the sawdust waste sample were Pseudomonas spp., Bacillus spp., Escherichia coli, Staphylococcus spp., klebsiella spp., Micrococcus spp., and Cellulomonas spp. While for the abattoir waste water, samples were Streptococcus spp., Pseudomonas spp., Staphylococcus aureus, Bacillus spp., Micrococcus spp.,Vibrio cholera, klebsiella spp., and Escherichia coli. The study concludes that microorganisms occur in a large number and variety in organic wastes.

TABLE OF CONTENTS
Certification ---    i
Dedication ---     ii
Acknowledgement ---    iii
Table of Contents ---    iv
List of Tables ---      vi
Abstract ---      vii

CHAPTER ONE
1.0 Introduction ---    1 
1.1 Justification of study ---      2
1.2 General objective of the study --- 3
1.3 Specific objectives of the study --- 3

CHAPTER TWO
2.0 Literature review --- 5
2.1 Organic wastes ---   5
2.2 Sources of organic waste  ---5 
2.2.1 Plant source ---  5
2.2.2 Animal source ---   5
2.3 Forms of organic waste ---  5 
2.3.1 Wet organic waste material   6
2.3.2   Dry organic waste material ---6
2.4 Categories of organic waste ---  6
2.4.1 Urban generated wastes ---  6
2.4.2 Animal wastes ---   7
2.5 Abattoir  ---    8
2.5.1  Activities that occur in abattoirs --- 9
2.5.2 Abattoir waste  ---   10 
2.6 Saw dust ---    10
2.6.1   Practical uses ---    11 

CHAPTER THREE
3.0 Materials and Methods ---   13
3.1  Study area  ---    13
3.2  Collection of samples ---   13
3.3  Media used and preparation ---  14
3.4  Enumeration of total heterotrophic bacteria from saw dust   14
3.5  Enumeration of total heterotrophic bacteria from abattoir waste water  15
3.6  Enumeration of cellulolytic bacteria from saw dust    15
3.7  Enumeration of cellulolytic bacteria from abattoir waste water  16
3.8  Identification and characterization of bacteria isolates    16
3.8.1  Microscopic examination ---  17
3.8.2  Gram staining  ---    17
3.8.3  Biochemical reaction tests ---  17

CHAPTER FOUR
4.0 Results --- 20

CHAPTER FIVE 
5.0 Discussion and Conclusion ---  26  
5.1  Discussion  ---    26
5.2  Conclusion ---    28
References

LIST OF TABLE
4.1 Microbial (bacterial) count of saw dust waste sample    22
4.2 Microbial  (bacterial) count of abattoir waste samples    23
4.3  Bacteria isolated from sawdust and their percentage occurrence  24
4.4  Bacteria isolated from abattoir waste water and their percentage occurrence 25

ISOLATION, CHARACTERIZATION AND IDENTIFICATION OF BACTERIA FROM ORGANIC WASTE
For more Info, call us on
+234 8130 686 500
or
+234 8093 423 853

Share This
  • Type: Project
  • Department: Microbiology
  • Project ID: MCB0258
  • Access Fee: ₦5,000 ($14)
  • Chapters: 5 Chapters
  • Pages: 48 Pages
  • Methodology: Scientific
  • Reference: YES
  • Format: Microsoft Word
  • Views: 1.3K
Payment Instruction
Bank payment for Nigerians, Make a payment of ₦ 5,000 to

Bank GTBANK
gtbank
Account Name Obiaks Business Venture
Account Number 0211074565

Bitcoin: Make a payment of 0.0005 to

Bitcoin(Btc)

btc wallet
Copy to clipboard Copy text

500
Leave a comment...

    Details

    Type Project
    Department Microbiology
    Project ID MCB0258
    Fee ₦5,000 ($14)
    Chapters 5 Chapters
    No of Pages 48 Pages
    Methodology Scientific
    Reference YES
    Format Microsoft Word

    Related Works

    ABSTRACT The isolation, identification and characterization of bacteria from organic waste were carried out. Sawdust waste (plant organic waste source) and abattoir waste water (animal organic waste source) samples were collected. The media used were nutrient agar for total aerobic plate count, McConkey agar for coliform count and cellulolytic... Continue Reading
    ABSTRACT The isolation, identification and characterization of bacteria from organic waste were carried out. Sawdust waste (plant organic waste source) and abattoir waste water (animal organic waste source) samples were collected. The media used were nutrient agar for total aerobic plate count, McConkey agar for coliform count and cellulolytic... Continue Reading
    ABSTRACT The consumption of Cow suya meat is on the increase because of it health benefits, it inexpensiveness compared to Goat meat, and the demand. Bacteriological analysis was carried out on 10 suya samples collected from four (4) different vendors within 4 different locations (Ekpo Abasi, Bogobiri, Yellow Duke and Abang asang). The samples... Continue Reading
    ABSTRACT There is a reasonable increase in the consumption of barbecue chicken meat, which is due to the it nutritional value. An investigation was conducted to isolate, characterize and identify bacteria associated with hawked barbecue chicken obtained from Calabar metropolis using basically two different agar media which are Nutrient agar and... Continue Reading
    ABSTRACT The isolation and identification of bacteria associated with 150ml sepsis studies were carried out to 250 patient at National Orthopedic Hospital Enugu. (250) Two hundred and fifty patients with 150ml sepstis was grouped in to in patients and out patients. The sample collected are wound pus and wound exudates which were collected from... Continue Reading
    ABSTRACT The isolation and identification of bacteria associated with  150ml sepsis studies were carried out to 250 patient at National Orthopedic Hospital Enugu. (250) Two hundred and fifty patients with 150ml sepstis was grouped in to in patients and out patients.  The  sample collected are wound pus and wound exudates which were collected... Continue Reading
    ABSTRACT The isolation and identification of bacteria associated with 150ml sepsis studies were carried out to 250 patient at National Orthopedic Hospital Enugu. (250) Two hundred and fifty patients with 150ml sepstis was grouped in to in patients and out patients. The sample... Continue Reading
      ABSTRACT The isolation and identification of bacteria associated with  150ml sepsis studies were carried out to 250 patient at National Orthopedic Hospital Enugu. (250) Two hundred and fifty patients with 150ml sepstis was grouped in to in patients and out patients.  The  sample collected are wound pus and wound exudates which were collected... Continue Reading
    (A Case Study Of Ogbete Market Enugu) ABSTRACT Safety of food is a basic requirement of food quality. A total of 25 street food samples (Jollof rice, egwusi soup, ugu, water leaf and green), were randomly purchased from five different vendors in Ogbete main... Continue Reading
    ABSTRACT The bacteria quality of four (4) commercially available dry sachet milk powder (peak, cowbell, Blue boat and Nunu) were analysed in this study usinf the pour plate method. The result showed that peak, cowbell and blue boat were free of all forms... Continue Reading
    Call Us
    Get this work
    whatsappWhatsApp Us