Antibacterial activity of honey obtained from two different locations in Enugu State (Nsukka & Ugwuaji) Nigeria on Staphylococcus aureus, Escherichia coli and Streptococcus pyogens isolated from wound was studied. Agar well diffusion method was used to determine the antibacterial activity of the honey on the test microorganisms. The result revealed that the two honey samples have heavy antibacterial activities against the test organisms and zones of inhibition were obtained showing high antibacterial activity. The antibacterial activity increased with increase in the concentrations and honey from Nsukka produced a high antibacterial activity (clearer zone) on staphylococcus aureus and Escherichia coli at all concentration and moderately for streptococcus pyogens. The use of honey as a therapeutic substance has been rediscovered by the medical profession on more recent times, and it is gaining acceptance as an antibacterial agent for the treatment of ulcers and bed sores, and other infections resulting from burns and wounds.
LIST OF TABLES
Table 1- Composition of honey13
Table 2- Result of Biochemical tests25
Table 3- Inhibition of honey from Enugu North (Nsukka)26
Table 4- Inhibition of honey from Enugu South (Ugwuaji)26
TABLE OF CONTENTS
Title Pagei
Certification Pageii
Dedicationiii
Acknowledgementiv
Table of Contentv
List of tablesviii
Abstractx
CHAPTER ONE
1.0Introduction1
1.1Aims and objectives3
CHAPTER TWO
2.0Literature review5
2.1 Wound infection8
2.2 Definition of honey9
2.3Local test for honey13
2.4Classification of honey14
2.5Preservation of honey16
2.6Properties and active ingredient of honey17
2.7Mode of action of some antibacterial substancepresent in honey20
2.8Clinical conditions for treatment with honey22
2.9Honey as an antimicrobial agent24
2.10Practical consideration for the clinical use of honey27
2.11Adverse reaction of honey28
2.12 Research on honey29
CHAPTER THREE
3.1Source of Sample32
3.2Sources of honey32
3.3Identification of organisms32
3.4Gram Staining33
3.5Indole test34
3.6Catalase test34
3.7Coagulase test35
3.8Antibacterial sensitivity test35
CHAPTER FOUR
4.1Result37
CHAPTER FIVE
5.1Discussion40
5.2Conclusion42
5.3Recommendation43
REFERENCE44
Appendix 151
Appendix 254
ANTIBACTERIAL ACTIVITY OF HONEY ON (STAPHYLOCOCCUS AUREUSESCHERICHIA COLI AND STREPTOCOCCUS PYOGEN) ISOLATED FROM WOUND
ABSTRACT Antibacterial activity of honey obtained from two different locations in Enugu State (Nsukka & Ugwuaji) Nigeria on Staphylococcus aureus, Escherichia coli and Streptococcus pyogens isolated from wound was studied. Agar well diffusion method was used to determine the antibacterial activity of the honey on the test microorganisms. The... Continue Reading
ABSTRACT Antibacterial activity of honey obtained from two different locations in Enugu State (Nsukka & Ugwuaji) Nigeria on Staphylococcus aureus, Escherichia coli and Streptococcus pyogens isolated from wound was studied. Agar well diffusion method was used to determine the antibacterial activity of the honey on the test microorganisms. The... Continue Reading
The growing threat and spread of antibiotic resistance by a wide range of common pathogens has led to increased investigations into traditional medicinal plants as alternatives. Honey has been used as a medicine since ancient times in many cultures and is still used in ‘folk medicine’. The moist environment of chronic wounds is an ideal growth... Continue Reading
ABSTRACT The antibacterial activity of sweet orange(citrus smensin) on staphylococcus aureus and Escherichia coli isolated from would infection was studied. A total number of 100 wound sample were collected form different individual from different age range within 5-8years and examined,. The test organisms S auras and E coli were cultured... Continue Reading
ABSTRACT The antibacterial activity of sweet orange(citrus smensin) on staphylococcus aureus and Escherichia coli isolated from would infection was studied. A total number of 100 wound sample were collected form... Continue Reading
ABSTRACT Two medicated soaps and one local soap were investigated for their antibacterial activities against Staphylococcus aureus . Identification of the bacteria species was by standard microbiological techniques which included colonial examination, gram staining, and biochemical testing. Minimum inhibitory and minimum bactericidal activities... Continue Reading
ABSTRACT This research work was carried out to examine the physicochemical and phytochemical constituents of honey and Shea butter samples respectively and their antibacterial effect on Staphylococcus aureus and Klebsiella pneumoniae . The physicochemical screening of honey showed that the honey samples used have low water activity, low moisture... Continue Reading
ABSTRACT This research work was carried out to examine the physicochemical and phytochemical constituents of honey and Shea butter samples respectively and their antibacterial effect on Staphylococcus aureus and Klebsiella pneumoniae . The physicochemical screening of honey showed that the honey samples used have low water activity, low moisture... Continue Reading
AND ESCHERICHIA COLI ISOLATED FROM WOUND INFECTED ABSTRACT The antibacterial activity of sweet orange(citrus smensin) on staphylococcus aureus and Escherichia coli isolated from would infection was studied. A total number of 100 wound sample were collected form... Continue Reading
ABSTRACT The antibacterial activity of three types of medicated soap on Staphylococcus aureus isolated from wound infections was conducted. Fourteen individuals with wound infections were sampled. Swab sticks were used to collect specimens from wound infections; each swab stick was streaked separately over plates of nutrient and mannitol agar and... Continue Reading