The purpose of this study was to examine the challenges training officers in the training department face in organizing In-Service Education and Training programmes in the Atwima Mponua District. A qualitative research design, specifically the case study design was employed for the study. Interview guide and observation were used to gather data from training officers in the District. A total of 14 training officers were used for the study using census sampling technique. Data collected was analyzed thematically. Findings from the study show that with regard to the challenges of In-Service Education and Training, the study revealed that there are inadequate funds to pay for snacks, furniture, stationery and others. Again, there is lack of resource materials and low commitment of teachers. It is recommended that Government, the Ghana Education Service, School Management Committee/Parent Teacher Association and Non-Governmental Organizations should ensure that there is adequate material and financial resources for the organization of InService Education and Training. The Ghana Education Service through the Training Officers should consider organizing school based In-Service Education and Training rather than circuit based ones to ensure good participation by most teachers.